Increased basal contractility of cardiomyocytes overexpressing protein kinase Cϵ and blunted positive inotropic response to endothelin-1

doi:10.1016/S0008-6363(01)00225-5

Increased basal contractility of cardiomyocytes overexpressing protein kinase Cϵ and blunted positive inotropic response to endothelin-1

^aDepartment of Cardiology and Angiology, University of Freiburg, D-79106 Freiburg, Germany
^bGeorg-August-Universitaet Goettingen, Zentrum Innere Medizin, Department of Cardiology and Pneumology, Robert-Koch-Strasse 40, D-37075 Goettingen, Germany
^cINSERM U533, F-44035 Nantes Cedex 01, France

* Corresponding author. Tel.: +49-551-396-380; fax: +49-551-392-953 stephane.baudet{at}intervet.com prestle{at}med.uni-goettingen.de

Received March 31, 2000.
Accepted January 11, 2001.

Abstract

Objective: Protein kinase C (PKC) is thought to be involved in the regulation of the mammalian cardiac excitation–contraction coupling process by vasoactive peptides like endothelin-1 (ET-1). However, the demonstration of a causal link between activation of specific PKC isoforms and the increase in contractility mediated by ET-1 is still inferential. Methods: By means of adenovirus-mediated gene transfer, we specifically overexpressed PKCϵ in cultured adult rabbit ventricular myocytes (Ad-PKCϵ). Myocyte shortening and [Ca²⁺]_i transients under basal and ET-1-stimulated conditions were measured in Ad-PKCϵ and Ad-LacZ control transfected cells. Results: Infection with Ad-PKCϵ resulted in a strong, virus dose-dependent increase in PKCϵ protein levels, whereas protein expression of other PKC isoforms remained unchanged. Using a multiplicity of infection of 100 plaque-forming units/myocyte, basal and cofactor-dependent PKCϵ kinase activity was increased 28- and 90-fold, respectively, when compared to control. Myocyte basal fractional shortening and [Ca²⁺]_i transient amplitude were both increased by 21% (P<0.05 each) in Ad-PKCϵ transfected myocytes when compared to Ad-LacZ transfected control myocytes. The positive inotropic effect of ET-1 in control myocytes was markedly blunted in PKCϵ-overexpressing myocytes. Conclusion: Specific overexpression of PKCϵ in rabbit ventricular myocytes increases basal myocyte contractility and [Ca²⁺]_i transients, and modifies their responsiveness to ET-1.