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Cardiovascular Research Advance Access [Accepted Manuscript] published online on April 7, 2009
Cardiovascular Research, doi:10.1093/cvr/cvp108
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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: journals.permissions@oxfordjournals.org.

Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress

Luciene Cristina Gastalho Campos, Ayumi Aurea Miyakawa, Valerio Garrone Barauna, Leandro Cardoso, Thaiz Ferraz Borin, Luis Alberto de Oliveira Dallan and Jose Eduardo Krieger

Heart Institute (InCor), University of Sao Paulo Medical School

Corresponding authors: AAM (ayumi.miyakawa{at}incor.usp.br) & JEK (krieger{at}incor.usp.br) Laboratory of Genetic and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Av. Dr. Eneas C. Aguiar, 44 – 10 andar, 05403-000 Sao Paulo – SP – Brazil, Tel.: 55 11 3069 5068, Fax: 55 11 3069 5022

Aims—: Cysteine- and glycine-rich protein 3/ muscle LIM-domain protein (CRP3/MLP) is a protein that mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodeling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins.

Methods and Results—: Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers.

Conclusion—: Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial hemodynamic conditions.

Time for primary review: 30 Days


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