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Cardiovascular Research Advance Access first published online on November 5, 2008
This version [Corrected Proof] published online on November 26, 2008
Cardiovascular Research, doi:10.1093/cvr/cvn302
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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2008. For permissions please email: journals.permissions@oxfordjournals.org.

Expression and vasoconstrictor function of anorexigenic peptides neuromedin U-25 and S in the human cardiovascular system

John D. Mitchell*, Janet J. Maguire, Rhoda E. Kuc and Anthony P. Davenport

Clinical Pharmacology Unit, Centre for Clinical Investigation, Box 110, Addenbrooke’s Hospital, University of Cambridge, Cambridge CB2 2QQ, UK

* Corresponding author. Tel: +44 1223 762564; fax: +44 1223 762576. E-mail address: jdm50{at}cam.ac.uk

Aims: Neuromedin U-25 (NMU-25), a brain–gut peptide with anorexigenic actions, was paired with the G-protein-coupled receptors NMU1 and NMU2 in 2000. NMU-25 elicited a potent hypertensive effect in rats but little is known about its cardiovascular effects in humans. We examined the hypothesis that NMU fulfils the criteria for controlling vascular reactivity within the human cardiovascular system.

Methods and results: The radioligand [125I]-NMU-25 demonstrated specific, saturable, and high affinity (KD = 0.26 ± 0.06 nM) binding in the human left ventricle and coronary artery, and quantitative reverse transcription-polymerase chain reaction revealed that mRNA encoding NMU1 predominated in these tissues. NMU-25-like immunoreactivity was detected in human plasma, left ventricle, coronary artery, saphenous vein, and epicardial adipose tissue, and both NMU-25 and a related peptide, neuromedin S (NMS), were identified by high-performance liquid chromatography in the left ventricle. NMU receptor and peptide were localized to endothelial cells, with the receptor also present on vascular smooth muscle cells. NMU-25 was a potent vasoconstrictor of isolated rings of human coronary and mammary artery and saphenous vein. Compared with NMU-25, NMS had a significantly reduced maximum response in saphenous vein, and the Arg165Trp variant of NMU-25, associated with childhood-onset obesity, was without effect. NMU-25 precursor mRNA was upregulated in the left ventricle from patients with dilated cardiomyopathy and ischaemic heart disease.

Conclusion: We have detected the expression of both NMU receptor and peptide in human cardiovascular tissues and have shown that NMU-25 and NMS act as potent vasoconstrictors in human vascular beds.

KEYWORDS Neuromedin U-25; Neuromedin S; Vasoconstriction; Human cardiovascular system; Arg165Trp amino-acid variant of neuromedin U-25

Time for primary review: 34 days


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