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Cardiovascular Research Advance Access first published online on January 4, 2008
This version [Corrected Proof] published online on February 1, 2008
Cardiovascular Research, doi:10.1093/cvr/cvm118
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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2007. For permissions please email: journals.permissions@oxfordjournals.org.

Tissue distribution and subcellular localization of the cardiac sodium channel during mouse heart development

Jorge N. Domínguez, Ángel de la Rosa, Francisco Navarro, Diego Franco and Amelia E. Aránega*

Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaén, Paraje de las Lagunillas, s/n, Jaén 23071, Spain

* Corresponding author. Tel: +34 953 212604; fax: +34 953 211875. E-mail address: aaranega{at}ujaen.es

Aims: The aim of this study was to analyse the mRNA expression levels and protein distribution of the cardiac sodium channel Scn5a/Nav1.5 during mouse cardiogenesis.

Methods and results: Scn5a mRNA levels were determined by real-time RT–PCR using embryonic hearts ranging from E9.5 to E17.5 as well as postnatal and adult hearts. In addition, Scn5a protein (Nav1.5) distribution was analysed by immunohistochemistry and confocal microscopy. Scn5a mRNA levels displayed a peak at stage E11.5, decreased during the subsequent stages and then steadily increased from E17.5 onwards, and throughout the postnatal to the adult stages. Immunohistochemistry experiments revealed comparable distribution of Nav1.5 between the different cardiac chambers at early embryonic stages. During the foetal stages, Nav1.5 showed an enhanced expression in the trabeculated myocardium and in the bundle branches. At the subcellular level, Nav1.5 and Scn1b double-immunostaining analysis is consistent with the presence of both sodium channel subunits in the T-tubule system and the intercalated discs.

Conclusion: Our results demonstrate that the cardiac sodium channel, Nav1.5, shows a dynamic expression pattern during mouse heart development, indicating that it could play an important role in the acquisition of a mature pattern of conduction and contraction during cardiogenesis.

KEYWORDS Ion channels; Gene expression; Cell differentiation

Time for primary review: 26 days


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