Skip Navigation


Cardiovascular Research Advance Access originally published online on July 16, 2008
Cardiovascular Research 2008 80(2):227-235; doi:10.1093/cvr/cvn192
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
80/2/227    most recent
cvn192v2
cvn192v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Liu, T.-J.
Right arrow Articles by Lai, H.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, T.-J.
Right arrow Articles by Lai, H.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2008. For permissions please email: journals.permissions@oxfordjournals.org

Ginkgo biloba extract 761 reduces doxorubicin-induced apoptotic damage in rat hearts and neonatal cardiomyocytes

Tsun-Jui Liu1,2,{dagger}, Yueh-Chiao Yeh1,{dagger}, Chih-Tai Ting1,2, Wen-Lieng Lee1,2, Li-Chuan Wang1, Hsiao-Wei Lee1, Kuo-Yang Wang1,3, Hui-Chun Lai4 and Hui-Chin Lai1,2,*

1 Cardiovascular Center, Taichung Veterans General Hospital, 160, Sec. 3, Taichungkang Road, Taichung 407, Taiwan, Republic of China
2 Department of Medicine, Surgery and Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan, Republic of China
3 Department of Medicine, Chung-Shan Medical University, Taichung, Taiwan, Republic of China
4 Chang-Gung Memorial Hospital and Chang-Gung University College of Medicine, Taoyuan, Linkoh, Taiwan, Republic of China

* Corresponding author. Tel: +886 4 23592525; fax: +886 4 23599257. E-mail address: 854k{at}vghtc.gov.tw; trliu{at}vghtc.gov.tw

Aims: The objective of this study was to investigate whether a cytoprotective herb-derived agent, Ginkgo biloba extract (EGb) 761, could have a beneficial effect on doxorubicin-induced cardiac toxicity in vitro and in vivo.

Methods and results: Primary cultured neonatal rat cardiomyocytes were treated with the vehicle, doxorubicin (1 µM), EGb761 (25 µg/mL), or EGb761 plus doxorubicin. After 24 h, doxorubicin upregulated p53 mRNA expression, disturbed Bcl-2 family protein balance, disrupted mitochondrial membrane potential, precipitated mitochondrion-dependent apoptotic signalling, induced apoptotic cell death, and reduced viability of cardiomyocytes, whereas EGb761 pretreatment suppressed all the actions of doxorubicin. Similarly, rats treated with doxorubicin [3 mg/kg intraperitoneally (i.p.) three doses every other day] displayed retarded growth of body and heart as well as elevated apoptotic indexes in heart tissue at both 7 and 28 days after exposure, whereas EGb761 pretreatment (5 mg/kg i.p. 1 day before each dose of doxorubicin) effectively neutralized the aforementioned gross and cellular adverse effects of doxorubicin.

Conclusion: Doxorubicin impairs viability of cardiomyocytes at least partially by activating the p53-mediated, mitochondrion-dependent apoptotic signalling. EGb761 can effectively and extensively counteract this action of doxorubicin, and may potentially protect the heart from the severe toxicity of doxorubicin.

KEYWORDS Ginkgo; Doxorubicin; Apoptosis; p53; Cardiomyocyte

Time for primary review: 30 days

{dagger} Tsun-Jui Liu and Yueh-Chiao Yeh contributed equally to this paper.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
H.-C. Ou, W.-J. Lee, I-T. Lee, T.-H. Chiu, K.-L. Tsai, C.-Y. Lin, and W. H.-H. Sheu
Ginkgo biloba extract attenuates oxLDL-induced oxidative functional damages in endothelial cells
J Appl Physiol, May 1, 2009; 106(5): 1674 - 1685.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.