Cardiovascular Research Advance Access originally published online on February 6, 2008
Cardiovascular Research 2008 78(2):395-403; doi:10.1093/cvr/cvn033
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Assessment of 
vβ3 integrin expression after myocardial infarction by positron emission tomography
1 Nuklearmedizinische Klinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, Ismaninger Straße 22, 81675 Munich, Germany
2 Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der Isar, Munich, Germany
3 Center of Integrated Protein Science in Department Chemie, Lehrstuhl II für Organische Chemie, Technische Universität München, Munich, Germany
4 Abteilung für Gefässchirurgie der Technischen Universität München, Klinikum rechts der Isar, Munich, Germany
* Corresponding author. Tel: +49 89 4140 2968; fax: +49 89 4140 4950. E-mail address: higuchi{at}po2.nsknet.or.jp
Aims: The purpose of this study was to determine the feasibility of a new positron emission tomography (PET) imaging approach using an 18F-labelled 
vβ3 integrin antagonist (18F-Galacto-RGD) to monitor the integrin expression after myocardial infarction.
Methods and results: Male Wister rats were subjected to 20 min transient left coronary artery occlusion followed by reperfusion. Autoradiographic analysis and in vivo PET imaging were used to determine myocardial 18F-Galacto-RGD uptake at different time points following reperfusion.
Results: PET imaging and autoradiography demonstrated no significant focal myocardial 18F-Galacto-RGD uptake in non-operated control rats and at day 1 after reperfusion. However, focal accumulation in the infarct area started at day 3 (uptake ratio = 1.91 ± 0.22 vs. remote myocardium), peaked between 1 (3.43 ± 0.57) and 3 weeks (3.43 ± 0.95), and decreased to 1.96 ± 0.40 at 6 months after reperfusion. Pretreatment with vβ3 integrin antagonist c(-RGDfV-) significantly decreased tracer uptake, indicating the specificity of tracer uptake. The time course of focal tracer uptake paralleled vascular density as measured by CD31 immunohistochemical analysis.
Conclusion: Regional 18F-Galacto-RGD accumulation suggests up-regulation of vβ3 integrin expression after myocardial infarction, which peaks between 1 and 3 weeks and remains detectable until 6 months after reperfusion. This new PET tracer is promising for the monitoring of myocardial repair processes.
KEYWORDS Angiogenesis; Infarction; Ischaemia; Reperfusion; Endothelial receptors
Time for primary review: 21 days
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Saraste, S. G. Nekolla, and M. Schwaiger Cardiovascular molecular imaging: an overview Cardiovasc Res, September 1, 2009; 83(4): 643 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Morooka, K. Kubota, H. Kadowaki, K. Ito, O. Okazaki, M. Kashida, T. Mitsumoto, R. Iwata, K. Ohtomo, and M. Hiroe 11C-Methionine PET of Acute Myocardial Infarction J. Nucl. Med., August 1, 2009; 50(8): 1283 - 1287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Laitinen, A. Saraste, E. Weidl, T. Poethko, A. W. Weber, S. G. Nekolla, P. Leppanen, S. Yla-Herttuala, G. Holzlwimmer, A. Walch, et al. Evaluation of {alpha}v{beta}3 Integrin-Targeted Positron Emission Tomography Tracer 18F-Galacto-RGD for Imaging of Vascular Inflammation in Atherosclerotic Mice Circ Cardiovasc Imaging, July 1, 2009; 2(4): 331 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Holz, R. Lautamaki, T. Sasano, J. Merrill, S. G. Nekolla, A. C. Lardo, and F. M. Bengel Expanding the Versatility of Cardiac PET/CT: Feasibility of Delayed Contrast Enhancement CT for Infarct Detection in a Porcine Model J. Nucl. Med., February 1, 2009; 50(2): 259 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Sinusas Targeted imaging offers advantages over physiological imaging for evaluation of angiogenic therapy. J. Am. Coll. Cardiol. Img., July 1, 2008; 1(4): 511 - 514. [Full Text] [PDF]
|
|