Skip Navigation

Cardiovascular Research 2007 75(1):178-185; doi:10.1016/j.cardiores.2007.03.028
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hao, X.
Right arrow Articles by Sylvén, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hao, X.
Right arrow Articles by Sylvén, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2007, European Society of Cardiology

Angiogenic effects of sequential release of VEGF-A165 and PDGF-BB with alginate hydrogels after myocardial infarction

Xiaojin Haoa, Eduardo A. Silvab, Agneta Månsson-Broberga, Karl-Henrik Grinnemoc, Anwar J. Siddiquia, Göran Dellgrenc, Eva Wärdella, Lars Åke Brodind, David J. Mooneyb and Christer Sylvéna,*

aKarolinska Institute at Department of Internal Medicine, Department of Cardiology, Karolinska University Hospital, Stockholm, Sweden
bSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
cKarolinska Institute at Department of Thoracic Surgery, Karolinska University Hospital, Stockholm, Sweden
dKarolinska Institute at Department of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden

* Corresponding author. Department of Cardiology, Karolinska University Hospital, S-141 86 Stockholm, Sweden. Tel.: +46 8 58580000; fax: +46 8 585 86710. Christer.Sylven{at}ki.se

Objective This study investigates whether local sequential delivery of vascular endothelial growth factor-A165 (VEGF-A165) followed by platelet-derived growth factor-BB (PDGF-BB) with alginate hydrogels could induce an angiogenic effect and functional improvement greater than single factors after myocardial infarction.

Methods Alginate hydrogels were prepared by combining high and low molecular weight alginate. Growth factor release rates were monitored over time in vitro with 125I-labelled VEGF-A165 and PDGF-BB included in the gels. One week after myocardial infarction was induced in Fisher rats, gels with VEGF-A165, PDGF-BB, or both were given intra-myocardially along the border of the myocardial infarction. Vessel density was analysed in hearts and cardiac function was determined by Tissue Doppler Echocardiography. In addition, the angiogenic effect of sequenced delivery was studied in vitro in aortic rings from C57B1/6 mice.

Results Alginate gels were capable of delivering VEGF-A165 and PDGF-BB in a sustainable manner, and PDGF-BB was released more slowly than VEGF-A165. Sequential growth factor administration led to a higher density of {alpha}-actin positive vessels than single factors, whereas no further increment was found in capillary density. Sequential protein delivery increased the systolic velocity-time integral and displayed a superior effect than single factors. In the aortic ring model, sequential delivery led to a higher angiogenic effect than single factor administration.

Conclusions The alginate hydrogel is an effective and promising injectable delivery system in a myocardial infarction model. Sequential growth factor delivery of VEGF-A165 and PDGF-BB induces mature vessels and improves cardiac function more than each factor singly. This may indicate clinical utility.

KEYWORDS Angiogenesis; Growth factors; Tissue engineering; Ventricular function; Infarction


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Am Coll CardiolHome page
J. Leor, S. Tuvia, V. Guetta, F. Manczur, D. Castel, U. Willenz, O. Petnehazy, N. Landa, M. S. Feinberg, E. Konen, et al.
Intracoronary injection of in situ forming alginate hydrogel reverses left ventricular remodeling after myocardial infarction in Swine.
J. Am. Coll. Cardiol., September 8, 2009; 54(11): 1014 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Zhang, L. Ding, Y. Zhao, W. Sun, B. Chen, H. Lin, X. Wang, L. Zhang, B. Xu, and J. Dai
Collagen-Targeting Vascular Endothelial Growth Factor Improves Cardiac Performance After Myocardial Infarction
Circulation, April 7, 2009; 119(13): 1776 - 1784.
[Abstract] [Full Text] [PDF]

Home page
 Eur J Heart FailHome page
T. Wang, D.-Q. Wu, X.-J. Jiang, X.-Z. Zhang, X.-Y. Li, J.-F. Zhang, Z.-B. Zheng, R. Zhuo, H. Jiang, and C. Huang
Novel thermosensitive hydrogel injection inhibits post-infarct ventricle remodelling
Eur J Heart Fail, January 1, 2009; 11(1): 14 - 19.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
N. Landa, L. Miller, M. S. Feinberg, R. Holbova, M. Shachar, I. Freeman, S. Cohen, and J. Leor
Effect of Injectable Alginate Implant on Cardiac Remodeling and Function After Recent and Old Infarcts in Rat
Circulation, March 18, 2008; 117(11): 1388 - 1396.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.