Copyright © 2006, European Society of Cardiology
Carbon monoxide released by CORM-3 inhibits human platelets by a mechanism independent of soluble guanylate cyclase
aDepartment of Experimental Pharmacology, Chair of Pharmacology, Jagiellonian University Medical College, 31-531 Krakow 16 Grzegorzecka, Poland
bVascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex, United Kingdom
* Corresponding author. Tel.: +48 12 421 11 68; fax: +48 12 421 72 17. Email address: s.chlopicki{at}cyfronet.krakow.pl
Objective Carbon monoxide (CO) modulates several physiological functions through activation of a cGMP-dependent pathway similar to that of nitric oxide (NO). Here we investigated the possible involvement of soluble guanylate cyclase in the anti-aggregatory effect of micromolar concentrations of CO released by a novel, water-soluble, CO releasing molecule (CORM) in human platelets.
Methods Human platelet aggregation was induced by collagen or thrombin, and the effects of CO releasing molecule (CORM-3) and an NO donor on platelet aggregation were compared.
Results CORM-3 liberated CO in a time- and concentration-dependent manner as evidenced by the formation of carbon monoxy myoglobin (MbCO) using a spectrophotometric assay. When added to washed platelets, CORM-3 (10–300 µM) inhibited collagen- and thrombin-induced aggregation in a concentration-dependent manner. The anti-aggregatory effect of CORM-3 was reversed by deoxy-Mb (50 µM). Interestingly, in the presence of an inhibitor of guanylate cyclase (ODQ, 5 µM), inhibition of collagen-induced aggregation by CORM-3 was not blocked but potentiated. Under the same experimental conditions, inhibition of platelet aggregation by an NO donor (SNAP, 1 µM) was prevented by ODQ. In collagen-induced or thrombin-induced platelet aggregation, a stimulator of guanylate cyclase (YC-1, 0.3 µM) did not alter the effect of CORM-3, whereas it markedly potentiated the inhibition of platelet aggregation mediated by SNAP. Notably, CORM-3-induced inhibition of platelet aggregation was of similar degree when platelets were activated by a low (20 mU/ml) or by high concentration of thrombin (100–200 mU/ml), whereas NO donors (SNP and SNAP)- or carbaprostacylin (cPGI2)-induced effects were considerably attenuated when platelets were activated by high concentrations of thrombin.
Conclusions Inhibition of platelet aggregation by CO released by a novel, water-soluble CORM is not mediated by activation of soluble guanylate cyclase. In contrast to NO and PGI2, CO effectively inhibits platelets even when cells are activated excessively. We suggest that despite the fact that CO is not a potent inhibitor of platelet activation, it may gain importance when NO and PGI2 alone are insufficient to overcome excessive platelet activation.
KEYWORDS Platelets; Carbon monoxide; Nitric oxide; Guanylate cyclase; Signal transduction; sGC; CORM-3; Vasoactive agents
Time for primary review 27 days
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Masini, A. Vannacci, P. Failli, R. Mastroianni, L. Giannini, M. C. Vinci, C. Uliva, R. Motterlini, and P. F. Mannaioni A carbon monoxide-releasing molecule (CORM-3) abrogates polymorphonuclear granulocyte-induced activation of endothelial cells and mast cells FASEB J, September 1, 2008; 22(9): 3380 - 3388. [Abstract] [Full Text] [PDF]
|
|