The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model

doi:10.1016/j.cardiores.2006.02.016

Cardiovascular Research 2006 70(3):530-542; doi:10.1016/j.cardiores.2006.02.016

This Article

	Full Text
	FREE Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Add to My Personal Archive
	Download to citation manager
	Request Permissions

Google Scholar

	Articles by Lim, S. Y.
	Articles by Park, J. C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lim, S. Y.
	Articles by Park, J. C.

Social Bookmarking

	What's this?

The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model

Sang Yup Lim^a^,1, Yong Sook Kim^a^,1, Youngkeun Ahn^a^,*, Myung Ho Jeong^a, Moon Hwa Hong^a, Soo Yeon Joo^a, Kwang Il Nam^b, Jeong Gwan Cho^a, Peter M. Kang^c and Jong Chun Park^a

^aDepartment of Cardiovascular Medicine, The Heart Center of Chonnam National University Hospital, 8 Hak Dong, Dong Ku, Gwangju 501-757, South Korea
^bResearch Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, South Korea
^cCardiovascular Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, USA

^* Corresponding author. Tel.: +82 62 220 4764; fax: +82 62 223 3105. Email address: cecilyk{at}hanmail.net

Objective This study was designed to examine whether mesenchymalstem cells (MSCs) transduced with Akt enhance cardiac repairafter transplantation into the ischemic porcine heart.

Methods MSCs isolated from porcine bone marrow and transducedwith myr-Akt were transplanted into porcine hearts after experimentalmyocardial infarction (MI) using intracoronary injection [GroupI, vehicle; Group II, MSCs; Group III, Akt-MSCs]. Myocardialsingle photon emission tomography (M-SPECT) was performed toassess myocardial function and the infarcted area. Pigs werealso sacrificed for immunohistochemical characterization andhistologic analysis. In addition, in vitro assays were performedto examine the resistance of Akt-MSCs to H₂O₂ stimulation.

Results Transplantation of MSCs into the ischemic porcine myocardium(Group II) increased the left ventricular ejection fraction( ${delta}$ LV EF; – 6.3±15.1% versus 0.5±6.4%, P<0.001)and decreased the ${delta}$ area of MI (6.8±5.6% versus –5.0±5.3%, P<0.001) compared with the vehicle control(Group I). Transplantation of MSCs transduced with myr-Akt (GroupIII) resulted in further improvement in ${delta}$ LV EF (– 6.3±15.1%versus 5.8±11.3%, P<0.001) and in ${delta}$ area of MI (6.8±5.6%versus – 17.0±7.6%, P<0.001). Akt-MSCs weremore resistant to apoptosis, and the levels of extracellularsignal-regulated protein kinase (ERK) activation and vascularendothelial growth factor (VEGF) were higher in H₂O₂-stimulatedAkt-MSCs.

Conclusion Cellular transplantation of Akt-MSCs further enhancesthe repair of injured myocardium compared to MSC transplantationalone by increasing the number of viable MSCs after cellulartransplantation.

KEYWORDS Mesenchymal stem cell; Akt; Myocardial infarction; Porcine

¹ These authors contributed equally to this work.

Time for primary review 29 days

Add to CiteULike CiteULike Add to Connotea Connotea Add to Del.icio.us Del.icio.us What's this?

This article has been cited by other articles:

T. Kucic, I. B. Copland, J. Cuerquis, D. L. Coutu, L. E. Chalifour, R. F. Gagnon, and J. Galipeau
Mesenchymal stromal cells genetically engineered to overexpress IGF-I enhance cell-based gene therapy of renal failure-induced anemia
Am J Physiol Renal Physiol, August 1, 2008; 295(2): F488 - F496.
[Abstract] [Full Text] [PDF]