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Cardiovascular Research 2005 66(3):594-600; doi:10.1016/j.cardiores.2005.01.013
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Copyright © 2005, European Society of Cardiology

Increased expression of endothelial lipase in rat models of hypertension

Yasushi Shimokawaa, Ken-ichi Hirataa,*, Tatsuro Ishidaa, Yoko Kojimaa, Nobutaka Inouea, Thomas Quertermousb and Mitsuhiro Yokoyamaa

aDivision of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan
bDonald W. Reynolds Cardiovascular Clinical Research Center, Division of Cardiovascular Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA

* Corresponding author. Tel.: +81 78 382 5846; fax: +81 78 382 5859. Email address: hiratak{at}med.kobe-u.ac.jp

Objective: To gain a better understanding of the involvement of endothelial lipase (EL) in vascular disease, we examined whether the EL expression is regulated in animal models of hypertension.

Methods: The rat cDNA homologue of EL was identified using reverse transcription-polymerase chain reaction. Cultured rat aortic smooth muscle cells were stimulated with angiotensin II (Ang II) and phorbol 12-myristate 13-acetate (PMA), and EL mRNA expression was analyzed by Northern blotting. EL mRNA levels in tissues from stroke-prone spontaneously hypertensive rats (SHR-SP) and Ang II-induced hypertensive rats were evaluated using RNase protection assays.

Results: Rat EL cDNA encoded a protein containing 493 amino acid residues including a signal peptide, and shares 91.9% and 80.9% sequence homology with murine and human EL, respectively. Northern blotting revealed that EL was expressed in a wide range of rat tissues. In cultured rat aortic smooth muscle cells, Ang II and PMA increased EL mRNA levels by 2.9- and 3.3-fold, respectively. In Ang II-induced hypertensive rats, EL expression was upregulated in the aorta, heart, and lung. In SHR-SP, EL expression was upregulated in the aorta and heart.

Conclusion: EL expression is increased in rat models of hypertension. Thus, EL might have a role in the local pathophysiology of vascular diseases.

KEYWORDS Lipid; Lipoprotein; Lipase; Cholesterol; Hypertension; Remodeling; Phospholipase

Time for primary review 22 days


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