© 2004 by European Society of Cardiology
Copyright © 2004, European Society of Cardiology
Coronary microembolization does not induce acute preconditioning against infarction in pigs—the role of adenosine
aInstitut für Pathophysiologie, Zentrum für Innere Medizin, Universitätsklinikum Essen, Hufelandstraße 55, 45122 Essen, Germany
bKlinik für Kardiologie, Zentrum Für Innere Medizin, Universitätsklinikum Essen, Hufelandstraße 55, 45122 Essen, Germany
* Corresponding author. Tel.: +49-201-723-4480; fax: +49-201-723-4481. Email address: gerd.heusch{at}uni-essen.de
Objective: After coronary microembolization (ME) adenosine is released from ischemic areas of the microembolized myocardium. This adenosine dilates vessels in adjacent nonembolized myocardium and increases coronary blood flow. For ischemic preconditioning (IP) to protect the myocardium against infarction, an increase in the interstitial adenosine concentration (iADO) prior to the subsequent ischemia/reperfusion is necessary. We hypothesized that the adenosine release after ME is sufficient to increase iADO and protect the myocardium against infarction from subsequent ischemia/reperfusion. We have therefore compared myocardial protection by either coronary microembolization or ischemic preconditioning prior to ischemia/reperfusion. Methods: In anesthetized pigs, the left anterior descending (LAD) was cannulated and perfused from an extracorporeal circuit. In 11 pigs, sustained ischemia was induced by 85% inflow reduction for 90 min (controls). Two other groups of pigs were subjected either to IP (n=8; 10-min ischemia/15-min reperfusion) or coronary ME (n=9; i.c. microspheres; 42 µm Ø; 3000·ml–1·min inflow) prior to sustained ischemia. Coronary venous adenosine concentration (vADO) and iADO (microdialysis) were measured. Infarct size was determined after 2-h reperfusion by triphenyl tetrazolium chloride staining. Results: In pigs subjected to IP, infarct size was reduced to 2.6±1.1% (mean±S.E.M.) vs. 17.0±3.2% in controls. iADO was increased from 2.4±1.3 to 13.1±5.8 µmol·l–1 during the reperfusion following IP. In pigs subjected to ME, at 10 min after ME, coronary blood flow (38.6±3.6 to 53.6±4.3 ml·min–1) and vADO (0.25±0.04 to 0.48±0.07 µmol·l–1) were increased. However, iADO (2.0±0.5 at baseline vs. 2.3±0.6 µmol·l–1 at 10 min after ME) did not increase. Infarct size induced by sustained ischemia following ME (22.5±5.2%) was above that of controls for any given subendocardial blood flow. Conclusion: ME released adenosine into the vasculature and increased coronary blood flow. The failure of iADO to increase with ME possibly explains the lack of protection against infarction after ME.
KEYWORDS Adenosine; Preconditioning; Microcirculation
Time for primary review 15 days
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