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Cardiovascular Research 2004 61(1):115-122; doi:10.1016/j.cardiores.2003.11.003
© 2004 by European Society of Cardiology
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Copyright © 2004, European Society of Cardiology

Preconditioning delays Ca2+-induced mitochondrial permeability transition

Laurent Argauda, Odile Gateau-Roescha, Lara Chalabreysseb, Ludovic Gomeza, Joseph Loufouata, Françoise Thivolet-Béjuib, Dominique Robertc and Michel Ovize*,a

aINSERM E0226, Laboratoire de Physiologie Lyon-Nord, Université Claude Bernard Lyon I, 8, Avenue Rockefeller, 69373 Lyon, France
bLaboratoire d'Anatomie Pathologique, Hôpital Louis Pradel, Lyon, France
cDépartement d'Urgence et de Réanimation Médicale, Hôpital Edouard Herriot, Lyon, France

* Corresponding author. Tel.: +33-4-7877-7074; fax: +33-4-7877-7175. laurent.argaud{at}chu-lyon.fr, ovize{at}rockefeller.univ-lyon1.fr

Objective: We investigated whether ischemic preconditioning (PC) may modify mitochondrial permeability transition (MPT) pore opening. Methods: In protocol 1, New Zealand White rabbits underwent either no intervention (sham group) or 10 min of ischemia followed by 5 min of reperfusion, preceded (PC) or not (C; control) by one episode of 5 min of ischemia and 5 min of reperfusion. Rabbits were pretreated by either saline or the MPT pore inhibitor cyclosporin A (CsA), or its non-immunosuppressive derivative Cs29 (10 mg/kg, IV bolus). Hearts were harvested and mitochondria isolated for further assessment of Ca2+-induced MPT using a Ca2+-sensitive micro-electrode. In protocol 2, C and PC hearts underwent 30 min of ischemia and 4 h of reperfusion. They were pretreated either by saline, CsA or Cs29, as in protocol 1. Infarct size was assessed by triphenyltetrazolium, and apoptosis by TUNEL staining. Results: In protocol 1, the Ca2+ overload required to induce MPT pore opening was significantly higher in PC than in C hearts. CsA and Cs29 significantly increased the Ca2+ overload required for MPT pore opening. In protocol 2, mean infarct size averaged 25% of the risk region in CsA/Cs29 treated hearts versus 15% in PC and 55% in controls (P<0.05 vs. C, P = ns vs. PC). Cardiomyocyte apoptosis was significantly reduced by PC and cyclosporin treatment with a mean apoptotic index of less than 2% in either group versus more than 11% in controls. Conclusion: This suggests that delayed opening of MPT pore may play a major role in ischemic PC.

KEYWORDS Preconditioning; Calcium (cellular); Mitochondria; Apoptosis; Necrosis

Time for primary review 22 days


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