© 2003 by European Society of Cardiology
Copyright © 2003, European Society of Cardiology
Presence of bone-marrow- and neural-crest-derived cells in intimal hyperplasia at the time of clinical in-stent restenosis
Department of Cardiology, Heart Center University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
*Corresponding author. Tel.: +49-228-287-6670; fax: +49-228-287-4983. Email address: gerhard.bauriedel{at}ukb.uni-bonn.de
Objective: Intralesional data of coronary target lesions following stent implantation are infrequent. In addition, there is ongoing controversy on the origin of neointimal cells. In this respect, several lines of evidence revealed bone-marrow-derived endothelial progenitor and dendritic cells (DCs) as well as neural-crest-derived cells (NCCs) to contribute to atherosclerosis. Therefore, the objective of the present study was to assess cellularity, cell type and origin of neointimal cells in in-stent restenosis (ISR). Methods: Atherectomy specimens from 17 patients with coronary in-stent restenosis (n = 10; time post-stenting 5±3 months) and with peripheral in-stent restenosis (n = 7; 7±3 months) versus those from 10 patients with primary lesions were immunohistochemically examined for the presence of the determinants CD34, AC133, S100, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), nerve growth factor receptor (NGFR) and 
-smooth muscle actin followed by computer-assisted morphometry. Results: In-stent restenosis probes consistently demonstrated homogeneous hypercellularity (942±318 cells/mm2) compared to de novo lesions (347±120 cells/mm2, P<0.001). -smooth muscle actin positive cells occupied 67% of intimal cells in in-stent restenosis. As a key finding, expression of endothelial progenitor cells (CD34: 7.1±2.5% positive/total cells vs. 0.6±0.7%, P<0.001; AC133: 7.0±3.4% vs. 1.0±0.7%, P<0.001), dendritic cells (S100: 9.8±5.6% vs. 1.4±1.1%, P<0.001) and neural-crest-derived cells (GFAP: 7.9±2.4% vs. 3.1±1.0%; NSE: 4.4±2.6% vs. 1.3±1.6%; NGFR: 4.2±2.5% vs. 1.1±0.7%; each P<0.001) was significantly increased in in-stent restenosis compared to primary lesions. Conclusions: Bone-marrow- and neural-crest-derived cells, the most dendritic cells, are consistently present in in-stent restenosis, whereas -smooth muscle actin positive cells constitute the largest intimal cell pool. Our data suggest the recruitment of primarily extravascular cells within neointima formation in human in-stent restenosis.
KEYWORDS Atherosclerosis; Histo(patho)logy; Restenosis; Stem cells; Stents
Time for primary review 23 days
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Angelini, M. Della Barbera, and G. Thiene Interventional procedures for atherothrombosis: pathology of retrieved material Heart, October 1, 2007; 93(10): 1301 - 1308. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohtani, K. Egashira, Y. Ihara, K. Nakano, K. Funakoshi, G. Zhao, M. Sata, and K. Sunagawa Angiotensin II Type 1 Receptor Blockade Attenuates In-Stent Restenosis by Inhibiting Inflammation and Progenitor Cells Hypertension, October 1, 2006; 48(4): 664 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yilmaz, J. Weber, I. Cicha, C. Stumpf, M. Klein, D. Raithel, W. G. Daniel, and C. D. Garlichs Decrease in Circulating Myeloid Dendritic Cell Precursors in Coronary Artery Disease J. Am. Coll. Cardiol., July 4, 2006; 48(1): 70 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Menasche You Can't Judge a Book by Its Cover Circulation, March 14, 2006; 113(10): 1275 - 1277. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hao, G. Gabbiani, E. Camenzind, M. Bacchetta, R. Virmani, and M.-L. Bochaton-Piallat Phenotypic Modulation of Intima and Media Smooth Muscle Cells in Fatal Cases of Coronary Artery Lesion Arterioscler Thromb Vasc Biol, February 1, 2006; 26(2): 326 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bauriedel, D. Skowasch, U. Welsch, and B. Luderitz Role of dendritic cells in specific atherosclerosis types Eur. Heart J., January 1, 2006; 27(1): 116 - 116. [Full Text] [PDF]
|
|
|
|
|
|
Y. V. Bobryshev Role of dendritic cells in specific atherosclerosis types: reply Eur. Heart J., January 1, 2006; 27(1): 116 - 117. [Full Text] [PDF]
|
|
|
|
|
|
D. Skowasch, S. Schrempf, N. Wernert, M. Steinmetz, A. Jabs, I. Tuleta, U. Welsch, C. J. Preusse, J. A. Likungu, A. Welz, et al. Cells of primarily extravalvular origin in degenerative aortic valves and bioprostheses Eur. Heart J., December 1, 2005; 26(23): 2576 - 2580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Skowasch, A Jabs, R Andrie, B Luderitz, and G Bauriedel Progression of native coronary plaques and in-stent restenosis are associated and predicted by increased pre-procedural C reactive protein Heart, April 1, 2005; 91(4): 535 - 536. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Levitzki PDGF receptor kinase inhibitors for the treatment of restenosis Cardiovasc Res, February 15, 2005; 65(3): 581 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bauriedel, D. Skowasch, A. Jabs, S. Kramer, R. Andrie, and B. Luderitz Circulating monocytes and late in-stent restenosis J. Am. Coll. Cardiol., August 18, 2004; 44(4): 936 - 936. [Full Text] [PDF]
|
|
|
|
|
|
D. Fukuda, K. Shimada, A. Tanaka, T. Kawarabayashi, M. Yoshiyama, and J. Yoshikawa Circulating monocytes and late in-stent restenosis: Reply J. Am. Coll. Cardiol., August 18, 2004; 44(4): 936 - 937. [Full Text] [PDF]
|
|
|
|
 |
|
 D. Skowasch, A. Jabs, B. Luderitz, and G. Bauriedel Bone Marrow-Derived Cells and Vascular Growth Circ. Res., April 30, 2004; 94(8): e71 - e71. [Full Text] [PDF]
|
|