Skip Navigation

Cardiovascular Research 2003 58(2):469-477; doi:10.1016/S0008-6363(03)00266-9
© 2003 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Griese, D. P.
Right arrow Articles by Riegger, G. A.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Griese, D. P.
Right arrow Articles by Riegger, G. A.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2003, European Society of Cardiology

Vascular gene delivery of anticoagulants by transplantation of retrovirally-transduced endothelial progenitor cells

Daniel P. Griesea,*, Stefan Achatza,1, Christian A. Batzlspergera,1, Ulrike G. Strauchb, Bernhard Grumbecka, Joachim Weila and Günter A.J. Rieggera

aKlinik und Poliklinik für Innere Medizin II, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93042 Regensburg, Germany
bKlinik und Poliklinik für Innere Medizin I, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93042 Regensburg, Germany

daniel.griese{at}klinik.uni-regensburg.de

* Corresponding author. Tel.: +49-941-944-7211; fax: +49-941-944-7213.

Objective: Recent studies have documented the presence of bone marrow-derived endothelial progenitor cells (EPC) in the circulation of several species. This study was designed to evaluate the use of engineered EPC for vascular gene delivery into angioplasty-induced arterial lesions. Methods and Results: EPC could easily be isolated from whole bone marrow and peripheral blood of adult rats. Differentiation was induced by culture on fibronectin in the presence of endothelial specific growth factors. Rat EPC shared several phenotypic and functional properties with mature endothelial cells. Recombinant retroviruses were generated encoding for the anticoagulants tissue-type plasminogen activator (tPA) and hirudin. Efficient (>90%) ex vivo gene transfer could be achieved resulting in high levels of transgene production. Engineered EPC were locally infused into freshly balloon-injured carotid arteries. Analysis of day 7 vessels showed 73±10% luminal coverage of the lesioned arterial bed with transduced EPC. Sustained secretion of both anticoagulants could be detected in organ cultures of explanted arteries. EPC seeding inhibited dilation of the injured arterial segment and prevented reduction of media thickness. However, rapid repopulation with EPC failed to attenuate neointima formation in this model. Conclusions: Peripheral blood and bone marrow can be used as source for endothelial lineage cells. Cultured EPC can be genetically engineered by retroviral gene transfer and serve as cellular vehicles for vascular gene and drug delivery of anticoagulants. Local transplantation of EPC attenuates reendothelialization of angioplasty-injured arteries but fails to inhibit neointima proliferation.

KEYWORDS Angioplasty; Anticoagulants; Cell isolation; Endothelial function; Gene therapy

1 Stefan Achatz and Christian A. Batzlsperger contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Vasc MedHome page
C Kalka and I. Baumgartner
Gene and stem cell therapy in peripheral arterial occlusive disease
Vascular Medicine, May 1, 2008; 13(2): 157 - 172.
[Abstract] [PDF]

Home page
 J Am Coll CardiolHome page
S. V. Pislaru, A. Harbuzariu, R. Gulati, T. Witt, N. P. Sandhu, R. D. Simari, and G. S. Sandhu
Magnetically Targeted Endothelial Cell Localization in Stented Vessels
J. Am. Coll. Cardiol., November 7, 2006; 48(9): 1839 - 1845.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
T. Marumo, H. Uchimura, M. Hayashi, K. Hishikawa, and T. Fujita
Aldosterone Impairs Bone Marrow-Derived Progenitor Cell Formation
Hypertension, September 1, 2006; 48(3): 490 - 496.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
V. J. Dzau, M. Gnecchi, A. S. Pachori, F. Morello, and L. G. Melo
Therapeutic Potential of Endothelial Progenitor Cells in Cardiovascular Diseases
Hypertension, July 1, 2005; 46(1): 7 - 18.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Q. Liu, Z.-Q. Chen, G. C. Bobustuc, J. M. McNatt, H. Segall, S. Pan, J. T. Willerson, and P. Zoldhelyi
Local Gene Transduction of Cyclooxygenase-1 Increases Blood Flow in Injured Atherosclerotic Rabbit Arteries
Circulation, April 12, 2005; 111(14): 1833 - 1840.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H. Uchimura, T. Marumo, O. Takase, H. Kawachi, F. Shimizu, M. Hayashi, T. Saruta, K. Hishikawa, and T. Fujita
Intrarenal Injection of Bone Marrow-Derived Angiogenic Cells Reduces Endothelial Injury and Mesangial Cell Activation in Experimental Glomerulonephritis
J. Am. Soc. Nephrol., April 1, 2005; 16(4): 997 - 1004.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.