Skip Navigation

Cardiovascular Research 2003 58(2):435-443; doi:10.1016/S0008-6363(02)00730-7
© 2003 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Johkura, K.
Right arrow Articles by Sasaki, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johkura, K.
Right arrow Articles by Sasaki, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2003, European Society of Cardiology

Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants

Kohei Johkuraa,*, Li Cuib, Akihiro Suzukia, Ruifeng Tengb, Akiko Kamiyoshic, Shintaro Okamuraa, Suguru Kubotab, Xu Zhaob, Kazuhiko Asanumaa, Yasumitsu Okouchia, Naoko Ogiwaraa, Yoh-ichi Tagawac and Katsunori Sasakia,b

aDepartment of Anatomy and Organ Technology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan
bInstitute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan
cInstitute of Experimental Animals, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

* Corresponding author. Tel.: +81-263-372590; fax: +81-263-373-093. kohei{at}sch.md.shinshu-u.ac.jp

Objective: Embryonic stem cell-derived cardiomyocytes are a useful source for cell transplantation into the heart, as well as for tissue engineering of the extracardiac vascular system. The present study was designed to investigate the survival and contractile function of embryonic stem cell-derived cardiomyocytes around large blood vessels to assess the feasibility of their ectopic use for future engineering of cardiovascular tissues. Methods: The mouse embryonic stem cell-derived cardiomyocytes were transplanted into the retroperitoneum of the adult nude mice, and the myocardial tissues that developed were characterized by electrophysiological and histological techniques. Results: Macroscopic and electrophysiological analyses showed spontaneously contracting transplants in the host retroperitoneum 7 and 30 days after transplantation. Immunohistochemistry detected developing cardiomyocytes in the transplants on Day 7, which formed the myocardial tissues. They were positive for cardiac troponin I, cadherin, connexin 43, and proliferating cell nuclear antigen, but negative for {alpha}-smooth muscle actin. Vascular formation was discernible in the transplant tissues. By Day 30, more mature myocardial tissues had been established in the transplants. Electron microscopic study emphasized that the transplant tissues comprised cardiomyocytes, in which myofibrils with organized sarcomeres were observed. Desmosomes, fasciae adherens and gap junctions were evident in the cellular junctions. Conclusions: The cardiomyocytes derived from the mouse ES cells were demonstrated to be viable and function in the ectopic site of the host retroperitoneum up to Day 30, following a process of proliferation and differentiation. Vascularization and host perfusion beneficial for the survival of the cardiomyocytes occurred in the transplants.

KEYWORDS Contractile function; Developmental biology; Histo(patho)logy; Myocytes; Stem cells; Transplantation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Circ. Res.Home page
J. Rajasingh, E. Bord, H. Hamada, E. Lambers, G. Qin, D. W. Losordo, and R. Kishore
STAT3-Dependent Mouse Embryonic Stem Cell Differentiation Into Cardiomyocytes: Analysis of Molecular Signaling and Therapeutic Efficacy of Cardiomyocyte Precommitted mES Transplantation in a Mouse Model of Myocardial Infarction
Circ. Res., October 26, 2007; 101(9): 910 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Yamamoto, L. Cui, K. Johkura, K. Asanuma, Y. Okouchi, N. Ogiwara, and K. Sasaki
Branching ducts similar to mesonephric ducts or ureteric buds in teratomas originating from mouse embryonic stem cells
Am J Physiol Renal Physiol, January 1, 2006; 290(1): F52 - F60.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R.-J. Swijnenburg, M. Tanaka, H. Vogel, J. Baker, T. Kofidis, F. Gunawan, D. R. Lebl, A. D. Caffarelli, J. L. de Bruin, E. V. Fedoseyeva, et al.
Embryonic Stem Cell Immunogenicity Increases Upon Differentiation After Transplantation Into Ischemic Myocardium
Circulation, August 30, 2005; 112(9_suppl): I-166 - I-172.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
L. Cui, K. Johkura, F. Yue, N. Ogiwara, Y. Okouchi, K. Asanuma, and K. Sasaki
Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation
J. Histochem. Cytochem., November 1, 2004; 52(11): 1447 - 1457.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.