© 2002 by European Society of Cardiology
Copyright © 2002, European Society of Cardiology
Expression of estrogen receptor subtypes and neuronal nitric oxide synthase in neutrophils from women and men
Regulation by estrogen
aCardiovascular Research Laboratory, Fundación Jiménez Díaz, Avda. Reyes Catolicos 2, Madrid 28040, Spain
bObstetrics and Gynecology Department, Fundación Jiménez Díaz, Madrid, Spain
* Corresponding author. Tel.: +34-91-550-4821; fax: +34-91-549-4764 alopeza{at}fjd.es
Objectives: (a) To identify the subtype of estrogen receptor (ER) expressed in neutrophils from premenopausal women and in neutrophils from men under different estrogen conditions and (b) to analyze the association between the modifications in the expression of ER subtypes and neuronal nitric oxide synthase (nNOS) expression induced by estrogen. Methods: Neutrophils were isolated from pre-menopausal women during different stages of the menstrual cycle and from ten men for in vitro estrogen incubations. Results: Neutrophils from premenopausal women expressed both ER
and ERβ subtypes which were increased in the ovulatory phase of the menstrual cycle. Neutrophils derived from men also expressed ER
and ERβ but only ER
expression was enhanced by in vitro incubation with 17β-estradiol (10–8 mol/l). In vitro incubation of neutrophils from women with 17β-estradiol enhanced expression of both ER-
and ER-β subtypes. nNOS protein was overexpressed in neutrophils from premenopausal women during the ovulatory phase. 17β-Estradiol (10–8 mol/l) also increased nNOS protein expression in neutrophils derived from men. Mithramycin A (10–6 mol/l) and curcumin (10–6 mol/l), prevented the upregulation of nNOS and ER
in neutrophils derived from men, suggesting the involvement of AP-1 and Sp-1 transcription factors. Conclusions: Although the in vivo levels of circulating estrogen concentrations seem to be associated with overexpression of both ER
and ERβ in neutrophils from premenopausal women, which was further confirmed by the in vitro experiments with neutrophils from women, in vitro incubation of neutrophils from men with 17β-estradiol only increased ER
protein expression which was associated with enhanced expression of nNOS protein.
KEYWORDS Gender; Gene expression; Leukocyte; Nitric Oxide; Receptors
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
D. Stygar, B. Masironi, H. Eriksson, and L. Sahlin Studies on estrogen receptor (ER) {alpha} and {beta} responses on gene regulation in peripheral blood leukocytes in vivo using selective ER agonists J. Endocrinol., July 1, 2007; 194(1): 101 - 119. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bouman, M. J. Heineman, and M. M. Faas Sex hormones and the immune response in humans Hum. Reprod. Update, July 1, 2005; 11(4): 411 - 423. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Liu, B. Xu, T. A. Cavalieri, and C. E. Hock Attenuation of antioxidative capacity enhances reperfusion injury in aged rat myocardium after MI/R Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2719 - H2727. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Sans In search of the grail: the never-ending story of biomarkers for coronary risk prediction Eur. Heart J., August 1, 2004; 25(15): 1271 - 1273. [Full Text] [PDF] |
||||
![]() |
D. Xing, A. Miller, L. Novak, R. Rocha, Y.-F. Chen, and S. Oparil Estradiol and Progestins Differentially Modulate Leukocyte Infiltration After Vascular Injury Circulation, January 20, 2004; 109(2): 234 - 241. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Liew, M. A Stagg, J. Chan, P. Collins, and K. T MacLeod Gender determines the acute actions of genistein on intracellular calcium regulation in the guinea-pig heart Cardiovasc Res, January 1, 2004; 61(1): 66 - 76. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. J. Molloy, A. J. O'Neill, J. J. Grantham, M. Sheridan-Pereira, J. M. Fitzpatrick, D. W. Webb, and R. W. G. Watson Sex-specific alterations in neutrophil apoptosis: the role of estradiol and progesterone Blood, October 1, 2003; 102(7): 2653 - 2659. [Abstract] [Full Text] [PDF] |
||||
![]() |
X.-J. Du Clues to understanding the role of estrogen receptors in mediating cardiovascular protection Cardiovasc Res, October 1, 2002; 56(1): 4 - 7. [Full Text] [PDF] |
||||






