© 2002 by European Society of Cardiology
Copyright © 2002, European Society of Cardiology
Lipopolysaccharide triggers late preconditioning against myocardial infarction via inducible nitric oxide synthase
aFirst Department of Surgery, Kagawa Medical University, 1750-1, Ikenobe, Miki, Kita, Kagawa, 761-0793, Japan
bDepartment of Molecular Biology, Kagawa Medical University, 1750-1, Ikenobe, Miki, Kita, Kagawa, 761-0793, Japan
* Corresponding author. Tel.: +81-878-912-186; fax: +81-878-912-187 wang{at}kms.ac.jp
Objective: The aim of this study was to investigate the role of inducible nitric oxide synthase (iNOS) as a trigger in lipopolysaccharide (LPS)-induced late preconditioning against myocardial infarction. Methods: Rats were pretreated intraperitoneally with two different doses of LPS (0.5 or 2.5 mg/kg) or normal saline (control) 24 h prior to lethal myocardial ischemia. Subsequently, all rats were subjected to a sustained 30-min coronary occlusion followed by 180-min reperfusion. In the second study, total RNA and protein were extracted from myocardium of the control and LPS-treated rats (after 4, 6 and 24 h of treatment) for reverse transcription-polymerase chain reaction and Western blot analysis. Results: In LPS (2.5 mg/kg, but not 0.5 mg/kg)-treated rats receiving no pharmacological intervention, the percentage of myocardial infarct within the area at risk and left ventricle was significantly reduced to 42±4 and 24±2% (P<0.01) compared with the control group. The cardioprotection was abolished by injection of dexamethasone (4 mg/kgx2, i.p.) or the selective iNOS inhibitor aminoguanidine (300 mg/kgx2, s.c.) before LPS treatment. The expression of iNOS mRNA and the iNOS protein significantly increased 4 and 6 h after administration of LPS (2.5 mg/kg), respectively. The increases in iNOS mRNA and protein were eliminated by dexamethasone. But the iNOS mRNA and protein were not detectable 24 h after administration of LPS (2.5 mg/kg). Conclusions: These data provide molecular and pharmacological evidence that LPS-induced late preconditioning against myocardial infarction is triggered by iNOS.
KEYWORDS Endotoxins; Gene expression; Infarction; Ischemia; Nitric oxide; Preconditioning
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