© 2002 by European Society of Cardiology
Copyright © 2002, European Society of Cardiology
Increased gene expression of tumor necrosis factor superfamily ligands in peripheral blood mononuclear cells during chronic heart failure
aResearch Institute for Internal Medicine, Rikshospitalet University Hospital, University of Oslo, N-0027 Oslo, Norway
bSection of Clinical Immunology and Infectious Diseases, Rikshospitalet University Hospital, University of Oslo, N-0027 Oslo, Norway
cDepartment of Cardiology, Rikshospitalet University Hospital, University of Oslo, N-0027 Oslo, Norway
dMSD Cardiovascular Research Centre, Rikshospitalet University Hospital, University of Oslo, N-0027 Oslo, Norway
eCentre for Occupational and Environmental Medicine, Rikshospitalet University Hospital, University of Oslo, N-0027 Oslo, Norway
fDepartment of Medicine, Bærum Hospital, Sandvika, Norway
* Corresponding author. Tel.: +47-23-073-629; fax: +47-23-073-630 arne.yndestad{at}klinmed.uio.no
Objective: Inflammation may play a pathogenic role in chronic heart failure (CHF). The objective of the study was to characterise the imbalance in the cytokine network in CHF. Methods: cDNA expression arrays were used to analyse the gene expression of cytokines and related mediators in peripheral blood mononuclear cells (PBMC) from CHF patients (n=8) and healthy controls (n=8). Real-time quantitative reverse transcription–polymerase chain reaction (RT-PCR) was used to determine the gene expression of individual genes in additional 12 patients and eight controls. Results: From 375 genes, 34 were upregulated and two downregulated in CHF patients in the cDNA expression array experiments. Regulated genes included chemokines/-receptors, members of the transforming growth factor β superfamily, orphan receptors and in particular several members of the tumor necrosis factor (TNF) superfamily. Thus, 4-1BB ligand (L), APRIL, CD27L, CD40L, FasL, LIGHT, TRAIL-receptor 4 were upregulated, while TRAIL-receptor 3 was downregulated. Real-time quantitative RT-PCR confirmed significantly upregulated gene expression of APRIL, LIGHT, FasL and CD27L in CHF patients and showed in addition significantly enhanced gene expression of TNF
and TRAIL. Conclusion: The present study demonstrates differential gene expression in PBMC of several members of the cytokine network in CHF. In particular, the enhanced expression of several ligands in the TNF superfamily may reflect a potential pathogenic role of these cytokines in CHF.
KEYWORDS Heart failure; Immunology; Cytokines; Leukocytes; Gene expression
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Cappuzzello, M. Napolitano, D. Arcelli, G. Melillo, R. Melchionna, L. Di Vito, D. Carlini, L. Silvestri, S. Brugaletta, G. Liuzzo, et al. Gene expression profiles in peripheral blood mononuclear cells of chronic heart failure patients Physiol Genomics, August 7, 2009; 38(3): 233 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Niessner, P. J. Hohensinner, K. Rychli, S. Neuhold, G. Zorn, B. Richter, M. Hulsmann, R. Berger, D. Mortl, K. Huber, et al. Prognostic value of apoptosis markers in advanced heart failure patients Eur. Heart J., April 1, 2009; 30(7): 789 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Dahl, L. Gullestad, B. Fevang, A. M. Holm, L. Landro, L. E. Vinge, A. E. Fiane, W. J. Sandberg, K. Otterdal, S. S. Froland, et al. Increased expression of LIGHT/TNFSF14 and its receptors in experimental and clinical heart failure Eur J Heart Fail, April 1, 2008; 10(4): 352 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sun, R. A. Ahokas, S. K. Bhattacharya, I. C. Gerling, L. D. Carbone, and K. T. Weber Oxidative stress in aldosteronism Cardiovasc Res, July 15, 2006; 71(2): 300 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Jankowska, S. von Haehling, A. Czarny, E. Zaczynska, A. Kus, S. D. Anker, W. Banasiak, and P. Ponikowski Activation of the NF-{kappa}B system in peripheral blood leukocytes from patients with chronic heart failure Eur J Heart Fail, October 1, 2005; 7(6): 984 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dyroy, A. Yndestad, T. Ueland, B. Halvorsen, J. K. Damas, P. Aukrust, and R. K. Berge Antiinflammatory Effects of Tetradecylthioacetic Acid Involve Both Peroxisome Proliferator-Activated Receptor {alpha}-Dependent and -Independent Pathways Arterioscler Thromb Vasc Biol, July 1, 2005; 25(7): 1364 - 1369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ueland, A. Yndestad, E. Oie, G. Florholmen, B. Halvorsen, S. S. Froland, S. Simonsen, G. Christensen, L. Gullestad, and P. Aukrust Dysregulated Osteoprotegerin/RANK Ligand/RANK Axis in Clinical and Experimental Heart Failure Circulation, May 17, 2005; 111(19): 2461 - 2468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Granger, T. Vowinkel, and T. Petnehazy Modulation of the Inflammatory Response in Cardiovascular Disease Hypertension, May 1, 2004; 43(5): 924 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Waehre, A. Yndestad, C. Smith, T. Haug, S. H. Tunheim, L. Gullestad, S. S. Froland, A. G. Semb, P. Aukrust, and J. K. Damas Increased Expression of Interleukin-1 in Coronary Artery Disease With Downregulatory Effects of HMG-CoA Reductase Inhibitors Circulation, April 27, 2004; 109(16): 1966 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yndestad, T. Ueland, E. Oie, G. Florholmen, B. Halvorsen, H. Attramadal, S. Simonsen, S. S. Froland, L. Gullestad, G. Christensen, et al. Elevated Levels of Activin A in Heart Failure: Potential Role in Myocardial Remodeling Circulation, March 23, 2004; 109(11): 1379 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Ahokas, K. J. Warrington, I. C. Gerling, Y. Sun, L. A. Wodi, P. A. Herring, L. Lu, S. K. Bhattacharya, A. E. Postlethwaite, and K. T. Weber Aldosteronism and Peripheral Blood Mononuclear Cell Activation: A Neuroendocrine-Immune Interface Circ. Res., November 14, 2003; 93 (10): e124 - e135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yndestad, A. M Holm, F. Muller, S. Simonsen, S. S Froland, L. Gullestad, and P. Aukrust Enhanced expression of inflammatory cytokines and activation markers in T-cells from patients with chronic heart failure Cardiovasc Res, October 15, 2003; 60(1): 141 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Scholz, A. Yndestad, J. K. Damas, T. Waehre, S. Tonstad, P. Aukrust, and B. Halvorsen 8-Isoprostane increases expression of interleukin-8 in human macrophages through activation of mitogen-activated protein kinases Cardiovasc Res, October 1, 2003; 59(4): 945 - 954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. Gerling, Y. Sun, R. A. Ahokas, L. A. Wodi, S. K. Bhattacharya, K. J. Warrington, A. E. Postlethwaite, and K. T. Weber Aldosteronism: an immunostimulatory state precedes proinflammatory/fibrogenic cardiac phenotype Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H813 - H821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Vasan, L. M. Sullivan, R. Roubenoff, C. A. Dinarello, T. Harris, E. J. Benjamin, D. B. Sawyer, D. Levy, P. W.F. Wilson, and R. B. D'Agostino Inflammatory Markers and Risk of Heart Failure in Elderly Subjects Without Prior Myocardial Infarction: The Framingham Heart Study Circulation, March 25, 2003; 107(11): 1486 - 1491. [Abstract] [Full Text] [PDF]
|
|