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Cardiovascular Research 2000 45(3):783-787; doi:10.1016/S0008-6363(99)00356-9
© 2000 by European Society of Cardiology
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Copyright © 2000, European Society of Cardiology

Antisense Bcl-x oligonucleotide induces apoptosis and prevents arterial neointimal formation in murine cardiac allografts

Jun-ichi Suzukia,*, Mitsuaki Isobea, Ryuichi Morishitab, Toshio Nishikawac, Jun Amanod and Yasufumi Kanedab

aDepartment of Internal Medicine I, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
bDivision of Gene Therapy Science, Osaka University Faculty of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
cDepartment of Pathology II, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan
dDepartment of Surgery II, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan

* Corresponding author. Present address: Vascular Medicine and Atherosclerosis Unit, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, 221 Longwood Ave, LMRC #307, Boston, MA 02115, USA. Tel.: +1-617-732-6628; fax: +1-617-732-6961

Objective: Cardiac allograft arteriosclerosis, which limits long-term survival of recipients, cannot be prevented by conservative therapies. The arteriopathy is characterized by diffuse intimal thickening comprised of proliferative smooth muscle cells (SMCs). Cell death is a prominent feature of atherosclerosis; Bcl-x is one of the anti-apoptotic mediators. Methods: To test the hypothesis that antisense bcl-x oligodeoxynucleotide (ODN) is effective in preventing intimal hyperplasia through enhancing apoptosis after cardiac transplantation, we performed single intraluminal delivery of antisense bcl-x ODN into murine cardiac allografts (n=9). DBA/2 (H-2d) hearts were transplanted into B10.D2 (H-2d) mice. Sense bcl-x ODN (n=8) and no treatment (n=8) studies were also performed. Results: Allografts were harvested at 4 weeks after transplantation; all allografts kept beating throughout the period. Coronary intimal thickening had developed in nontreated and sense ODN transfected allografts at 4 weeks after transplantation with enhanced expression of Bcl-x and cell adhesion molecules, and suppressed apoptosis. However, antisense bcl-x ODN prevented neointimal formation through enhanced apoptosis. Conclusion: These results indicate that apoptosis of vascular SMCs induced by Bcl-x is associated with initial hyperplasia after heart transplantation. Antisense bcl-x ODN inhibits SMC proliferation by inducing apoptosis in graft coronary arteries.

KEYWORDS Apoptosis; Transplantation


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