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Cardiovascular Research 1999 43(2):492-499; doi:10.1016/S0008-6363(99)00108-X
© 1999 by European Society of Cardiology
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Copyright © 1999, European Society of Cardiology

The isoprostane, 8-epi-PGF2{alpha}, is accumulated in coronary arteries isolated from patients with coronary heart disease

Mohammad Reza Mehrabia,*, Cem Ekmekcioglub, Franz Tatzberc, Anthony Oguoghoc, Robert Ullrichd, Abdelmonem Morgana, Forouzan Tamaddona, Michael Grimme, Helmut D Glogara and Helmut Sinzingerc

aDepartment of Cardiology, University of Vienna, Wahringer Gurtel 18-20, 1090 Vienna, Austria
bDepartment of Medical Physiology, University of Vienna, Vienna, Austria
cDepartment of Nuclear Medicine, University of Vienna, Vienna, Austria
dDepartment of Clinical Pathology, University of Vienna, Vienna, Austria
eDepartment of Surgery, University of Vienna, Vienna, Austria

* Corresponding author. Tel.: +43-140-4004614; fax: +43-140-81148 mmehrabi{at}pop3.kard.akh-wien.ac.at

Objective: In the present study we wanted to know whether 8-epi-PGF2{alpha}, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n=23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n=19) or from healthy heart donors (controls, n=6). Methods: Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2{alpha} in the vascular intima and media. In addition, the 8-epi-PGF2{alpha} content was determined using a specific immunoassay after extraction and purification. Results: The immunohistochemical results indicated that 8-epi-PGF2{alpha} is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P<0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too. Conclusions: From our findings we conclude that 8-epi-PGF2{alpha} is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis.

KEYWORDS Atherosclerosis; Coronary disease; Free radicals; Cardiomyopathy; Prostaglandins


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