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Cardiovascular Research 1999 41(1):246-254; doi:10.1016/S0008-6363(98)00231-4
© 1999 by European Society of Cardiology
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Copyright © 1999, European Society of Cardiology

Rabbit mononuclear leukocytes cause contraction of isolated aorta by the release of serotonin

Joanne L. Hart-Favaloro and Owen L. Woodman*

Department of Pharmacology, University of Melbourne, Parkville, Victoria 3052, Australia

* Corresponding author. Fax: +61-3-9347-1452; e-mail: o.woodman@pharmacology.unimelb.edu.au

Objective: The aim of this study was to examine the vasoactive effects of rabbit isolated mononuclear leukocytes, and to identify the mediators responsible for those vasoactive effects. Methods: Mononuclear leukocytes (MNLs) were isolated from New Zealand White rabbit whole blood, suspended at 5x107 cells/ml and incubated for 30 min at 37°C. This cell suspension, or the cell-free supernatant from this suspension, were then examined for vasoactive effects in rabbit isolated thoracic aorta. Results: Both the MNL suspension and the cell-free supernatant from this suspension caused endothelium-independent contraction of aortic rings, both from resting tension and when pre-contracted. The MNL suspension caused a significantly greater contraction than the MNL supernatant under all conditions. The contractions to the MNL product were significantly inhibited by the 5-HT2 receptor antagonist ketanserin (0.1 µM), but not by the {alpha}1-adrenoceptor antagonist prazosin (10 µM). High-performance liquid chromatography (HPLC) analysis showed that the MNL supernatant contained serotonin (5-hydroxytryptamine, 5-HT) at an average concentration of 5 µM. Conclusions: We conclude that MNLs cause contraction of rabbit isolated aortic rings by the release of 5-HT.

KEYWORDS Leukocyte; Monocyte; Lymphocyte; Vasocontraction; Rabbit, aorta; Serotonin


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