© 1998 by European Society of Cardiology
Copyright © 1998, European Society of Cardiology
Nuclear factor NF-
B in myocardium: developmental expression of subunits and activation by interleukin-1β in cardiac myocytes in vitro
Cardiothoracic Surgery, Imperial College School of Medicine, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK
* Corresponding author. Tel.: +44 (0)171 351 8184; Fax: +44 (0)171 376 3442; E-mail: p.barton@ic.ac.uk
Objective: The aims of the study were to investigate the pattern of expression of the major subunits of the NF-
B transcription factor complex in human and rat heart development, and to characterise the timing of NF-B activation by interleukin-1β (IL-1β) in rat neonatal cardiac myocytes. Methods: The expression of NF-B subunits p65 and p50 and the inhibitory subunits IB-
and IB-β in human and rat myocardial samples was measured by immunoblotting, using antibodies specific to each subunit. The activation of NF-B was measured in neonatal rat cardiac myocytes that were treated with IL-1β for different times (0–60 min). Depletion of the inhibitory factors IB- and IB-β was assessed by immunoblotting. The presence of NF-B DNA binding activity was measured directly in nuclear extracts by electrophoretic mobility shift assay (EMSA). Results: p65, p50, IB- and IB-β are expressed at all stages of development analysed. In human myocardial samples, expression of p50, p65 and IB- show an apparent gradual decline relative to total protein. In contrast, the level of IB-β remained relatively constant, suggesting a significant shift in the ratio of β and subunits with development. In rat myocardium, p65, p50, IB- and IB-β showed a gradual decline during development, with a particularly pronounced decrease between the ten day post-natal and adult samples. Treatment of neonatal rat cardiac myocytes with IL-1β (5 ng/ml) caused a rapid and transient depletion of IB- (reducing to 16±1.6% of initial levels within 5 min, returning to 82±10% within 60 min). A slower, less marked depletion is observed for IB-β (24±6% by 30 min, returning to only 49±5% by 60 min). Rapid and transitory accumulation of NF-B DNA binding activity was detected in the nucleus, with a pattern that correlated with the depletion of IB-. Conclusions: The principal NF-B subunits p65, p50, IB- and IB-β are present throughout development, suggesting that this transcription complex may participate in myocardial gene regulation throughout development and in the adult. Activation by IL-1β demonstrates that NF-B probably plays a direct role in the regulation of gene transcription in response to cytokine activation in cardiac myocytes.
KEYWORDS Development; Cardiac myocyte; NF-B; Interleukin-1β; Rat; Human
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Cuenca, N. Goren, P. Prieto, P. Martin-Sanz, and L. Bosca Selective Impairment of Nuclear Factor-{kappa}B-Dependent Gene Transcription in Adult Cardiomyocytes: Relevance for the Regulation of the Inflammatory Response in the Heart Am. J. Pathol., September 1, 2007; 171(3): 820 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Frantz, J. Tillmanns, P. J. Kuhlencordt, I. Schmidt, A. Adamek, C. Dienesch, T. Thum, S. Gerondakis, G. Ertl, and J. Bauersachs Tissue-Specific Effects of the Nuclear Factor {kappa}B Subunit p50 on Myocardial Ischemia-Reperfusion Injury Am. J. Pathol., August 1, 2007; 171(2): 507 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Frantz, K. Hu, B. Bayer, S. Gerondakis, J. Strotmann, A. Adamek, G. Ertl, and J. Bauersachs Absence of NF-{kappa}B subunit p50 improves heart failure after myocardial infarction FASEB J, September 1, 2006; 20(11): 1918 - 1920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Goren, J. Cuenca, P. Martin-Sanz, and L. Bosca Attenuation of NF-{kappa}B signalling in rat cardiomyocytes at birth restricts the induction of inflammatory genes Cardiovasc Res, November 1, 2004; 64(2): 289 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Suzuki, B. Murtuza, J. R. Beauchamp, N. J. Brand, P. J. R. Barton, A. Varela-Carver, S. Fukushima, S. R. Coppen, T. A. Partridge, and M. H. Yacoub Role of Interleukin-1{beta} in Acute Inflammation and Graft Death After Cell Transplantation to the Heart Circulation, September 14, 2004; 110(11_suppl_1): II-219 - II-224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Frantz, D. Fraccarollo, H. Wagner, T. M Behr, P. Jung, C. E Angermann, G. Ertl, and J. Bauersachs Sustained activation of nuclear factor kappa B and activator protein 1 in chronic heart failure Cardiovasc Res, March 1, 2003; 57(3): 749 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wright, I. S. Singh, J. D. Hasday, I. K. Farrance, G. Hall, A. S. Cross, and T. B. Rogers Endotoxin stress-response in cardiomyocytes: NF-kappa B activation and tumor necrosis factor-alpha expression Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H872 - H879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Takano, T. Nagai, M. Asakawa, T. Toyozaki, T. Oka, I. Komuro, T. Saito, and Y. Masuda Peroxisome Proliferator-Activated Receptor Activators Inhibit Lipopolysaccharide-Induced Tumor Necrosis Factor-{alpha} Expression in Neonatal Rat Cardiac Myocytes Circ. Res., September 29, 2000; 87(7): 596 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamamoto, Q. N. Dang, S. P. Kennedy, R. Osathanondh, R. A. Kelly, and R. T. Lee Induction of Tenascin-C in Cardiac Myocytes by Mechanical Deformation. ROLE OF REACTIVE OXYGEN SPECIES J. Biol. Chem., July 30, 1999; 274(31): 21840 - 21846. [Abstract] [Full Text] [PDF]
|
|