© 1998 by European Society of Cardiology
Copyright © 1998, European Society of Cardiology
K+-induced dilation of a small renal artery: no role for inward rectifier K+ channels
Department of Pharmacology, University of Leeds, Leeds, LS2 9JT, UK
* Corresponding author. Tel.: (+44-113) 2334310; Fax: (+44-113) 2334331; E-mail: d.j.beech@leeds.ac.uk
Objective: To investigate the mechanism of K+-induced vasodilation in a small artery from the kidney, with a particular emphasis on the role of inward rectifier K+ channels. Methods: Lumen diameter and isometric tension recordings have been made from rabbit renal arcuate artery using pressurised- and wire-myography respectively. In addition, conventional whole-cell and amphotericin-perforated patch whole-cell recordings have been made from single smooth muscle cells isolated from the vessel. Results: Arcuate arteries dilated when the extracellular K+ concentration was raised to 8–10 mM from either zero or a normal physiological level of about 6 mM. The effect was not endothelium-dependent. Application of 0.01–1 mM Ba2+ to block inward rectifier K+ channels had no significant effect on K+-induced vasodilation in the arcuate artery, but under the same experimental conditions K+-induced dilation of the rat posterior cerebral artery was abolished by Ba2+. In the presence of 60 mM extracellular K+, inward rectifier K+-current was detectable in some single smooth muscle cells isolated from arcuate arteries but on average the current density was low (–1.44 pA pF–1 at –60 mV). K+-induced vasodilation of the arcuate artery was abolished by 10 µM ouabain and the half-effective concentration of K+ which induced vasodilation was 0.9–1.5 mM. Conclusions: The observations suggest that an increase in the extracellular K+ concentration (up to about 10 mM) dilates the rabbit renal arcuate artery and that the primary mechanism underlying the effect may be stimulation of Na+–K+ ATPase in the smooth muscle cell membrane. Inward rectifier K+ channels have a low average density in smooth muscle cells isolated from arcuate arteries and play no significant role in K+-induced vasodilation.
KEYWORDS Vasodilation; Arcuate artery; Rabbit; Kidney; K+ channel; Na+–K+ ATPase
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