© 1997 by European Society of Cardiology
Copyright © 1997, European Society of Cardiology
Effects of 
-adrenergic stimulation on the sarcolemmal Na+/Ca2+-exchanger in adult rat ventricular cardiocytes
aUniversität Freiburg, Innere Medizin III, Kardiologie und Angiologie, Freiburg, Germany
bMax-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin-Buch, Germany
* Corresponding author. Present address: University of Washington, Department of Pathology, Box 357470, Room E520 HSB, Seattle, WA 98195-7335, Tel. (+1-206) 6168684; Fax (+1-206) 5433644; E-mail: hreineck@u.washington.edu
Objective: The cardiac sarcolemmal Na+/Ca2+-exchanger (NCX) plays an important role in the maintenance of the myocardial Ca2+ homeostasis which is altered in cardiac hypertrophy and failure. The aim of the present study was to investigate whether 
-adrenergic stimulation known to induce cardiac hypertrophy might be involved in the regulation of the sarcolemmal NCX. Methods: Adult rat ventricular cardiocytes (ARC) were isolated from male Sprague–Dawley rats. Phenylephrine, an -adrenergic agonist, was used as hypertrophic agent. NCX expression was measured by competitive RT-PCR and Western blot analysis. Results: -Adrenergic stimulation of ARC with 10 µM phenylephrine for 24 h resulted in a significant increase of the NCX mRNA (2.5-fold) and the NCX protein level (1.8-fold). The changes on the expression level were blocked by the 1-adrenoceptor antagonist prazosin. Conclusions: The data demonstrate that the NCX expression level is up-regulated by the activation of the -adrenergic signal transduction pathway. The increased NCX mRNA level induced by -adrenergic stimulation appeared to be translated into an increased NCX protein level.
KEYWORDS Na+/Ca2+-exchanger; Rat; Cardiocytes; -adrenoceptor; Expression
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