© 1997 by European Society of Cardiology
Copyright © 1997, European Society of Cardiology
Adenovirus gene therapy for hypercholesterolemia, thrombosis and restenosis
Center For Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Katholieke Universiteit Leuven, Campus Gasthuisberg, O&N, Herestraat 49, B-3000 Leuven, Belgium
* Corresponding author. Tel.: +32 16 346182; Fax: +32 16 345990.
Received 20 February 1997; accepted 1 May 1997
KEYWORDS Adenovirus vector; Vascular disease
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1 General properties of recombinant adenovirus vectors |
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Human adenoviruses are non-enveloped, icosahedral viruses, approximately 130 nm in diameter. The linear, double-stranded DNA viral genome containing covalently attached terminal protein derived from a unique replication process is almost 36 Kb in length. Although forty nine serotypes of human adenovirus in six serological subgroups have been identified, only serotypes 2 and 5 of subgroup C have been extensively employed as gene transfer vectors.
Recombinant helper-independent adenovirus vectors are easily constructed, analyzed and propagated using standard recombinant DNA and virological techniques (reviewed in [1, 2]). Foreign genes can be inserted into the adenovirus genome in a variety of locations to generate recombinant vectors, although substitution of early region 1 (E1) has been most widely used. Replacement of E1, since it is required for the efficient expression of the remainder of the viral genome, generates a vector which can only be propagated in a complementing cell line which supply the
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2 Adenovirus infection in vivo |
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2.1 Systemic gene transfer into liver
2.2 Local gene transfer into the vessel wall
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3 Adenovirus gene therapy of pathologic processes |
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3.1 Hypercholesterolemia
3.2 Thrombosis
3.3 Restenosis
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4 A perspective on the use of adenovirus vectors for gene therapy |
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