Skip Navigation

Cardiovascular Research 1997 33(3):655-665; doi:10.1016/S0008-6363(96)00264-7
© 1997 by European Society of Cardiology
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Haller, C.
Right arrow Articles by Kübler, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haller, C.
Right arrow Articles by Kübler, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 1997, European Society of Cardiology

Cytotoxicity of radiocontrast agents on polarized renal epithelial cell monolayers

Christlieb Haller*, Cornelia S. Schick, Markus Zorn and Wolfgang Kübler

Department of Internal Medicine III (Cardiology), University of Heidelberg, Bergheimerstr. 58, D-69115 Heidelberg, Germany

Objective: Radiocontrast-induced nephropathy is a clinically important complication of coronary angiography. The cellular mechanisms of radiocontrast-induced renal dysfunction are not clear. Since tubular transport functions depend on the polarity of renal epithelial cells, we investigated the effects of radiocontrast agents on polarized tubular cells in vitro. Methods: We studied the effects of iso-iodine concentrations (37 and 74 mg iodine/ml) of an ionic (diatrizoate) and a non-ionic (iopamidol) monomeric radiocontrast agent and of hyperosmolal mannitol control solutions on filter-grown renal epithelial cell (MDCK, LLCPK) monolayers in vitro. The cytotoxicity was assayed by measurement of cell viability, transepithelial resistance, inulin permeability and (polarized) cellular enzyme release. The polarized MDCK cell phenotype was assessed by transmission electron microscopy and indirect immunofluorescence microscopy using monoclonal antibodies against specific apical (gp135) and basal (gp60, uvomorulin) MDCK surface markers. Results: The radiocontrast agents reduced cell viability to a greater extent than hyperosmolal mannitol solutions in both cell lines; diatrizoate was more toxic than iopamidol. LLCPK cells were more susceptible to radiocontrast cytotoxicity than MDCK cells. This cytotoxicity was associated with an alteration of MDCK cell polarity as assessed by the redistribution of surface marker proteins. Conclusions: Diatrizoate is more toxic than iopamidol, which is partly related to its higher osmolality. The cytotoxicity of radiocontrast agents induces a redistribution of polarized membrane proteins which could contribute to the pathophysiology of radiocontrast-induced nephropathy.

KEYWORDS MEM = minimal essential medium; DMEM = Dulbecco's modified Eagle medium; LDH = lactate dehydrogenase; GGT = {gamma}-glutamyl transferase; AP = alkaline phosphatase; β-NAG = β-N-acetylglucosaminidase; PBS = phosphate-buffered saline

* Corresponding author. Tel. +49 6221 568676; Fax +49 6221 565515. challer{at}krzmail.krz.uni-heidelberg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eur Heart JHome page
G. Romano, C. Briguori, C. Quintavalle, C. Zanca, N. V. Rivera, A. Colombo, and G. Condorelli
Contrast agents and renal cell apoptosis
Eur. Heart J., October 2, 2008; 29(20): 2569 - 2576.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
G. T. C. Wong and M. G. Irwin
Contrast-induced nephropathy
Br. J. Anaesth., October 1, 2007; 99(4): 474 - 483.
[Abstract] [Full Text] [PDF]

Home page
 RadiologyHome page
M. C. Heinrich, M. K. Kuhlmann, S. Kohlbacher, M. Scheer, A. Grgic, M. B. Heckmann, and M. Uder
Cytotoxicity of Iodinated and Gadolinium-based Contrast Agents in Renal Tubular Cells at Angiographic Concentrations: In Vitro Study
Radiology, December 19, 2006; (2006) 2422060245.
[Abstract] [Full Text]

Home page
 RadiologyHome page
M. C. Heinrich, M. K. Kuhlmann, A. Grgic, M. Heckmann, B. Kramann, and M. Uder
Cytotoxic Effects of Ionic High-osmolar, Nonionic Monomeric, and Nonionic Iso-osmolar Dimeric Iodinated Contrast Media on Renal Tubular Cells in Vitro
Radiology, June 1, 2005; 235(3): 843 - 849.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Sera, H. Fujioka, H. Yokota, A. Makinouchi, R. Himeno, R. C. Schroter, and K. Tanishita
Localized compliance of small airways in excised rat lungs using microfocal X-ray computed tomography
J Appl Physiol, May 1, 2004; 96(5): 1665 - 1673.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Radiol.Home page
N F Fanning, B J Manning, J Buckley, and H P Redmond
Iodinated contrast media induce neutrophil apoptosis through a mitochondrial and caspase mediated pathway
Br. J. Radiol., November 1, 2002; 75(899): 861 - 873.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Radiol.Home page
A D Blann, R Adams, R Ashleigh, S Naser, U Kirkpatrick, and C N McCollum
Changes in endothelial, leucocyte and platelet markers following contrast medium injection during angiography in patients with peripheral artery disease
Br. J. Radiol., September 1, 2001; 74(885): 811 - 817.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.