© 1995 by European Society of Cardiology
Copyright © 1995, European Society of Cardiology
Effects of nitric oxide synthase inhibition on the muscle blood flow response to exercise in rats with heart failure
aDepartments of Anatomy and Physiology, Kinesiology, Kansas State University, Manhattan, KS, 66506, USA
bDepartments of Medicine, Cellular and Molecular Physiology, The Pennsylvania State University, Hershey, PA 17033, USA
* Corresponding author. Tel. (+913) 532-4523; Fax (+913) 532-4557.
Objective: The aim of this study was to investigate whether the role of nitric oxide (NO) in regulating blood flow (BF) to working skeletal muscle is impaired in chronic heart failure (CHF). Methods: The effect of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthesis, was studied in conscious rats with and without CHF due to myocardial infarction (MI). BF to the hindquarter musculature was measured with radiolabelled microspheres during exercise after 4 min of treadmill running (10% grade, 20 m/min) before and after L-NAME (20 mg/kg i.a.) administration. Results: Before L-NAME administration, BF measured in the total hindquarter musculature was less (P < 0.05) during exercise in rats with a large MI (MI size; 44 ± 2% of the left ventricular endocardial circumference; n = 8) when compared with sham-operated rats (SHAM; n = 10) and rats with a small MI (MI size; 25 ± 4%; n = 5). The BF measured during exercise following L-NAME administration was similar between the 3 groups. Of the 28 individual hindquarter muscles, BF was reduced in 23 and 19 muscles following the administration of L-NAME for the SHAM rats and rats with a small MI, respectively. In comparison, BF was reduced to only 4 of 28 muscles in rats with a large MI. Conclusions: These results suggest that the contribution of the NO pathway to the hyperaemic BF responses found in the hindquarter muscles during exercise could be attenuated in rats with CHF. This attenuation of the NO pathway may be associated with the impairment of skeletal muscle BF distribution during exercise in CHF.
KEYWORDS EDRF; L-NAME; Heart failure; Skeletal muscle blood flow; Rat, skeletal muscle
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