Cardiovascular Research Advance Access first published online on October 19, 2009
This version [Corrected Proof] published online on November 11, 2009
Cardiovascular Research, doi:10.1093/cvr/cvp344
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Proteomic remodelling of mitochondrial oxidative pathways in pressure overload-induced heart failure
1 Department of Cardiac Surgery, University of Leipzig Heart Center, Strümpellstr. 39, Leipzig 04289, Germany
2 Program in Molecular Medicine and Division of Endocrinology, Metabolism and Diabetes, Salt Lake City, UT, USA
3 Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA
4 Department of Nutrition and Food Sciences and Center for Integrated BioSystems, Utah State University, Logan, UT, USA
* Corresponding author. Tel: +49 341 865 1423, Fax: +49 341 865 1452, Email: torsten.doenst{at}med.uni-leipzig.de
Aims: Impairment in mitochondrial energetics is a common observation in animal models of heart failure, the underlying mechanisms of which remain incompletely understood. It was our objective to investigate whether changes in mitochondrial protein levels may explain impairment in mitochondrial oxidative capacity in pressure overload-induced heart failure.
Methods and results: Twenty weeks following aortic constriction, Sprague-Dawley rats developed contractile dysfunction with clinical signs of heart failure. Comparative mitochondrial proteomics using label-free proteome expression analysis (LC-MS/MS) revealed decreased mitochondrial abundance of fatty acid oxidation proteins (six of 11 proteins detected), increased levels of pyruvate dehydrogenase subunits, and upregulation of two tricarboxylic acid cycle proteins. Regulation of mitochondrial electron transport chain subunits was variable, with downregulation of 53% of proteins and upregulation of 25% of proteins. Mitochondrial state 3 respiration was markedly decreased independent of the substrate used (palmitoyl-carnitine –65%, pyruvate –75%, glutamate –75%, dinitrophenol –82%; all P < 0.05), associated with impaired mitochondrial cristae morphology in failing hearts. Perfusion of isolated working failing hearts showed markedly reduced oleate (–68%; P < 0.05) and glucose oxidation (–64%; P < 0.05).
Conclusion: Pressure overload-induced heart failure is characterized by a substantial defect in cardiac oxidative capacity, at least in part due to a mitochondrial defect downstream of substrate-specific pathways. Numerous changes in mitochondrial protein levels have been detected, and the contribution of these to oxidative defects and impaired cardiac energetics in failing hearts is discussed.
KEYWORDS Chronic heart failure; Pressure overload; Metabolism; Mitochondria; Proteomic remodelling
Time for primary review: 42 days
Present address. Department of Cardiology, University of Freiburg, Freiburg, Germany.
Present address. Department of Population Health and Reproduction, University of California, Davis, School of Veterinary Medicine, Davis, CA, USA.