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Cardiovascular Research Advance Access first published online on July 17, 2009
This version [Corrected Proof] published online on August 14, 2009
Cardiovascular Research, doi:10.1093/cvr/cvp246
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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: journals.permissions@oxfordjournals.org.

Redox modification of ryanodine receptors underlies calcium alternans in a canine model of sudden cardiac death

Andriy E. Belevych1, Dmitry Terentyev1, Serge Viatchenko-Karpinski1, Radmila Terentyeva1, Arun Sridhar1,2, Yoshinori Nishijima1,2, Lance D. Wilson3, Arturo J. Cardounel4, Kenneth R. Laurita3, Cynthia A. Carnes1,2, George E. Billman1 and Sandor Gyorke1,*

1 Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, College of Medicine, 473 W. 12th Avenue, Columbus, OH 43210, USA
2 College of Pharmacy, The Ohio State University, Columbus, OH, USA
3 The Heart and Vascular Research Center, Case Western Reserve University, MetroHealth Campus, Cleveland, OH, USA
4 Department of Physiology, College of Medicine, University of Florida, Gainesville, FL, USA

* Corresponding author. Tel: +1 614 292 3969; fax: +1 614 247 7799. E-mail address: sandor.gyorke{at}osumc.edu

Aims: Although cardiac alternans is a known predictor of lethal arrhythmias, its underlying causes remain largely undefined in disease settings. The potential role of, and mechanisms responsible for, beat-to-beat alternations in the amplitude of systolic Ca2+ transients (Ca2+ alternans) was investigated in a canine post-myocardial infarction (MI) model of sudden cardiac death (SCD).

Methods and results: Post-MI dogs had preserved left ventricular (LV) function and susceptibility to ventricular fibrillation (VF) during exercise. LV wedge preparations from VF dogs were more susceptible to action potential (AP) alternans and the frequency-dependence of Ca2+ alternans was shifted towards slower rates in myocytes isolated from VF dogs relative to controls. In both groups of cells, cytosolic Ca2+ transients ([Ca2+]c) alternated in phase with changes in diastolic Ca2+ in sarcoplasmic reticulum ([Ca2+]SR), but the dependence of [Ca2+]c amplitude on [Ca2+]SR was steeper in VF cells. Abnormal ryanodine receptor (RyR) function in VF cells was indicated by increased fractional Ca2+ release for a given amplitude of Ca2+ current and elevated diastolic RyR-mediated SR Ca2+ leak. SR Ca2+ uptake activity did not differ between VF and control cells. VF myocytes had an increased rate of reactive oxygen species production and increased RyR oxidation. Treatment of VF myocytes with reducing agents normalized parameters of Ca2+ handling and shifted the threshold of Ca2+ alternans to higher frequencies.

Conclusion: Redox modulation of RyRs promotes generation of Ca2+ alternans by enhancing the steepness of the Ca2+ release–load relationship and thereby providing a substrate for post-MI arrhythmias.

KEYWORDS Cardiac alternans; Ryanodine receptor; Redox modification; Ca2+ release; Myocardial infarction

Time for primary review: 28 days


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