Cardiovascular Research Advance Access first published online on November 29, 2008
This version [Corrected Proof] published online on December 21, 2008
Cardiovascular Research, doi:10.1093/cvr/cvn336
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
JNK1/c-fos inhibits cardiomyocyte TNF-
expression via a negative crosstalk with ERK and p38 MAPK in endotoxaemia
1 Department of Medicine, University of Western Ontario, London, Ontario, Canada N6A 5C1
2 Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada N6A 5C1
3 Centre for Critical Illness Research, Lawson Health Research Institute, London, Ontario, Canada
* Corresponding author. Tel: +1 519 850 2989; fax: +1 519 661 4051. E-mail address: qfeng{at}uwo.ca
Aims: Myocardial tumour necrosis factor-alpha (TNF-
) production plays an important role in cardiac dysfunction during sepsis. The aim of this study was to investigate the role of c-Jun NH2-terminal kinases (JNK) signalling in cardiomyocyte TNF- expression during lipopolysaccharide (LPS) stimulation and myocardial function in endotoxaemic mice.
Methods and results: In cultured neonatal mouse cardiomyocytes, deficiency of JNK1 or selective inhibition of JNK1 signalling by over-expression of a dominant negative mutant of JNK1 enhanced LPS-induced TNF- expression, which was associated with elevations in phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and p38 mitogen-activated protein kinase (MAPK). At the organ level, LPS-induced TNF- expression was significantly increased in JNK1–/– compared with wild-type hearts. JNK1 activation by LPS also induced immediate c-fos expression in cardiomyocytes, which was blocked by inhibition of JNK1 signalling. The role of c-fos expression in LPS-induced TNF- expression was investigated in both cultured c-fos–/– cardiomyocytes and isolated c-fos–/– hearts. Deficiency of c-fos significantly enhanced LPS-induced TNF- expression in cardiomyocytes and isolated hearts. Over-expression of c-fos decreased TNF- expression in LPS-stimulated cardiomyocytes, which was associated with a decrease in phosphorylation of ERK1/2 and p38. In mice with endotoxaemia, deficiency of either JNK1 or c-fos further decreased cardiac function compared with corresponding wild-type controls.
Conclusion: JNK1/c-fos inhibits ERK1/2 and p38 MAPK signalling, leading to decreased cardiomyocyte TNF- expression and improvements in cardiac function during endotoxaemia.
KEYWORDS Sepsis; Cytokine; Signal transduction; Cardiomyocytes; Cardiac function
Time for primary review: 23 days
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F.-L. Xiang, X. Lu, L. Hammoud, P. Zhu, P. Chidiac, J. Robbins, and Q. Feng Cardiomyocyte-Specific Overexpression of Human Stem Cell Factor Improves Cardiac Function and Survival After Myocardial Infarction in Mice Circulation, September 22, 2009; 120(12): 1065 - 1074. [Abstract] [Full Text] [PDF]
|
|