Skip Navigation


Cardiovascular Research Advance Access first published online on April 23, 2008
This version [Corrected Proof] published online on May 2, 2008
Cardiovascular Research, doi:10.1093/cvr/cvn097
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
79/3/395    most recent
cvn097v2
cvn097v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Aharinejad, S.
Right arrow Articles by Stanley, E. R.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aharinejad, S.
Right arrow Articles by Stanley, E. R.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2008. For permissions please email: journals.permissions@oxfordjournals.org

Colony-stimulating factor-1 transfection of myoblasts improves the repair of failing myocardium following autologous myoblast transplantation

Seyedhossein Aharinejad1,2,*, Dietmar Abraham2, Patrick Paulus2, Karin Zins2, Michael Hofmann2, Wolfgang Michlits2, Mariann Gyöngyösi3, Karin Macfelda4, Trevor Lucas2, Karola Trescher4, Michael Grimm1 and E. Richard Stanley5

1 Department of Cardio-Thoracic Surgery, Medical University of Vienna, Waehringerguertel 18–20, A-1090 Vienna, Austria
2 Laboratory for Cardiovascular Research, Department of Anatomy and Cell Biology, Medical University of Vienna, Waehringerstrasse 13, A-1090 Vienna, Austria
3 Department of Cardiology, Medical University of Vienna, Waehringerguertel 18–20, A-1090 Vienna, Austria
4 Center for Biomedical Research, Medical University of Vienna, Währingerguertel 18–20, A-1090, Vienna, Austria
5 Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA

* Corresponding author. Tel: +431 4277 61119; fax: +431 4277 61120. E-mail address: seyedhossein.aharinejad{at}meduniwien.ac.at

Aims: Skeletal myoblasts are used in repair of ischaemic myocardium. However, a large fraction of grafted myoblasts degenerate upon engraftment. Colony-stimulating factor-1 (CSF-1) accelerates myoblast proliferation and angiogenesis. We hypothesized that CSF-1 overexpression improves myoblast survival and cardiac function in ischaemia-induced heart failure.

Methods and results: Three weeks following myocardial infarction, rats developed heart failure and received intramyocardial injections of mouse CSF-1-transfected or untransfected primary autologous rat myoblasts, recombinant human CSF-1, mouse CSF-1 expressing plasmids, or culture medium. Tissue gene and protein expression was measured by quantitative RT–PCR (reverse transcription–polymerase chain reaction) and western blotting. Fluorescence imaging and immunocytochemistry were used to analyse myoblasts, endothelial cells, macrophages, and infarct wall thickening. Electrocardiograms were recorded online using a telemetry system. Left ventricular function was assessed by echocardiography over time, and improved significantly only in the CSF-1-overexpressing myoblast group. CSF-1-overexpression enhanced myoblast numbers and was associated with an increased infarct wall thickness, enhanced angiogenesis, increased macrophage recruitment and upregulated matrix metalloproteases (MMP)-2 and -12 in the zone bordering the infarction. Transplantation of CSF-1-overexpressing myoblasts did not result in major arrhythmias.

Conclusion: Autologous intramyocardial transplantation of CSF-1 overexpressing myoblasts might be a novel strategy in the treatment of ischaemia-induced heart failure.

KEYWORDS Heart failure; Myoblasts; Growth factor; Gene therapy

Time for primary review: 15 days


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?

Related Article

Towards the second generation of skeletal myoblasts?
Philippe Menasché
Cardiovasc Res 2008 79: 355-356. [Extract] [Full Text] [PDF]





Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.