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Cardiovascular Research 2007 76(3):517-527; doi:10.1016/j.cardiores.2007.08.007
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Copyright © 2007, European Society of Cardiology

Plasticity of CD133+ cells: Role in pulmonary vascular remodeling

Marta Díez, Joan A. Barberà, Elisabet Ferrer, Raquel Fernàndez-Lloris, Sandra Pizarro, Josep Roca and Victor I. Peinado*

Department of Pulmonary Medicine, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Ciber de Enfermedades Respiratorias, Universitat de Barcelona, Barcelona, Spain

*Corresponding author. Servei de Pneumologia, Hospital Clínic. Villarroel, 170, 08036 Barcelona, Spain. Tel.: +34 93 2275540; fax: +34 93 2275455. vpeinado{at}clinic.ub.es

Objective Studies in pulmonary arteries (PA) of patients with chronic obstructive pulmonary disease (COPD) suggest that bone marrow-derived endothelial progenitor cells (CD133+) may infiltrate the intima and differentiate into smooth muscle cells (SMC). This study aimed to evaluate the plasticity of CD133+ cells to differentiate into SMC and endothelial cells (EC) in both cell culture and human isolated PA.

Methods Plasticity of granulocyte-colony stimulator factor (G-CSF)-mobilized peripheral blood CD133+ cells was assessed in co-cultures with primary lines of human PA endothelial cells (PAEC) or SMC (PASMC) and in isolated human PA. We also evaluated if the phenotype of differentiated progenitor cells was acquired by fusion or differentiation.

Results The in vitro studies demonstrated CD133+ cells may acquire the morphology and phenotype of the cells they were co-cultured with. CD133+ cells co-incubated with human isolated PA were able to migrate into the intima and differentiate into SMC. Progenitor cell differentiation was produced without fusion with mature cells.

Conclusions We provide evidence of plasticity of CD133+ cells to differentiate into both endothelial cells and SMC, reinforcing the idea of their potential role in the remodeling process of PA in COPD. This process was conducted by transdifferentiation and not by cell fusion.

KEYWORDS Stem cells; Pulmonary circulation; Hypertension; Cell differentiation; Endothelial function


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