Copyright © 2007, European Society of Cardiology
Early electrical remodeling in rabbit pulmonary vein results from trafficking of intracellular SK2 channels to membrane sites
aCenter for Molecular Therapeutics, College of Physicians and Surgeons of Columbia University, New York, New York, United States
bDepartment of Pharmacology, College of Physicians and Surgeons of Columbia University, New York, New York, United States
cDepartment of Pediatrics, College of Physicians and Surgeons of Columbia University, New York, New York, United States
* Corresponding author. College of Physicians and Surgeons of Columbia University, Department of Pharmacology, 630 West 168 Street, PH 7West-321, New York, NY 10032, United States. Tel.: +1 212 305 8754; fax: +1 212 305 8351. mrr1{at}columbia.edu
Objective Atrial fibrillation is often initiated by bursts of ectopic activity arising in the pulmonary veins. We have previously shown that a 3-h intermittent burst pacing protocol (BPP), mimicking ectopic pulmonary vein foci, shortens action potential duration (APD) locally at the pulmonary vein–atrial interface (PV) while having no effect elsewhere in rabbit atrium. This shortening is Ca2+ dependent and is prevented by apamin, which blocks small conductance Ca2+-activated K+ channels (SKCa). The present study investigates the ionic and molecular mechanisms whereby two apamin-sensitive SKCa channels, SK2 and SK3, might contribute to the regional APD changes.
Methods Microelectrode and patch clamp techniques were used to record APDs and apamin-sensitive currents in isolated rabbit left atria and cells dispersed from PV and Bachmann's bundle (BB) regions. SK2 and SK3 mRNA and protein levels were quantified, and immunofluorescence was used to observe channel protein distribution.
Results There was a direct relationship between APD shortening and apamin-sensitive current in burst-paced but not sham-paced PV. Moreover, apamin-sensitive current density increased in PV but not BB after BPP. SK2 mRNA, protein, and current were increased in PV after BPP, while SK2 immunostaining shifted from a perinuclear pattern in sham atria to predominance at sites near or at the PV membrane.
Conclusions BPP-induced acceleration of repolarization in PV results from SK2 channel trafficking to the membrane, leading to increased apamin-sensitive outward current. This is the first indication of involvement of Ca2+-activated K+ currents in atrial remodeling and provides a possible basis for evolution of an arrhythmogenic substrate.
KEYWORDS Arrhythmias; Ion channels; K channels
1 Contributed equally to this study.
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