Copyright © 2007, European Society of Cardiology
Life history of eNOS: Partners and pathways
aDepartment of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States
bDepartment of Medicine, Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, United States
* Corresponding author. Tel.: +1 617 732 7376; fax: +1 617 732 5132. tmichel{at}rics.bwh.harvard.edu
The complex regulation of eNOS (endothelial nitric oxide synthase) in cardiovascular physiology occurs at multiple stages. eNOS mRNA levels are controlled both at the transcriptional and post-transcriptional phases, and epigenetic mechanisms appear to modulate tissue-specific eNOS expression. The eNOS enzyme reversibly associates with a diverse family of protein partners that regulate eNOS sub-cellular localization, catalytic function, and biological activity. eNOS enzyme activity and sub-cellular localization are intimately controlled by post-translational modifications including phosphorylation, nitrosylation, and acylation. The multiple extra-cellular stimuli affecting eNOS function coordinate their efforts through these key modifications to dynamically control eNOS and NO bioactivity in the vessel wall. This review will focus on the biochemical partners and perturbations of the eNOS protein as this vital enzyme undergoes modulation by diverse signal transduction pathways in the vascular endothelium.
KEYWORDS Nitric oxide synthase; Post-translational modifications; Sub-cellular targeting; Protein phosphorylation; Protein acylation; S-nitrosylation
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
C. N. Hess, R. Kou, R. P. Johnson, G. K. Li, and T. Michel ADP Signaling in Vascular Endothelial Cells: ADP-DEPENDENT ACTIVATION OF THE ENDOTHELIAL ISOFORM OF NITRIC-OXIDE SYNTHASE REQUIRES THE EXPRESSION BUT NOT THE KINASE ACTIVITY OF AMP-ACTIVATED PROTEIN KINASE J. Biol. Chem., November 20, 2009; 284(47): 32209 - 32224. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Stangl and V. Stangl The ubiquitin proteasome pathway and endothelial (dys)function Cardiovasc Res, October 13, 2009; (2009) cvp315v2. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Sennoun, C. Baron-Menguy, M. Burban, T. Lecompte, R. Andriantsitohaina, D. Henrion, A. Mercat, P. Asfar, B. Levy, and F. Meziani Recombinant human activated protein C improves endotoxemia-induced endothelial dysfunction: a blood-free model in isolated mouse arteries Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H277 - H282. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. R. Durrant, D. R. Seals, M. L. Connell, M. J. Russell, B. R. Lawson, B. J. Folian, A. J. Donato, and L. A. Lesniewski Voluntary wheel running restores endothelial function in conduit arteries of old mice: direct evidence for reduced oxidative stress, increased superoxide dismutase activity and down-regulation of NADPH oxidase J. Physiol., July 1, 2009; 587(13): 3271 - 3285. [Abstract] [Full Text] [PDF] |
||||
![]() |
J.-L. Balligand, O. Feron, and C. Dessy eNOS Activation by Physical Forces: From Short-Term Regulation of Contraction to Chronic Remodeling of Cardiovascular Tissues Physiol Rev, April 1, 2009; 89(2): 481 - 534. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Liu, Y. Gao, S. Negash, L. D. Longo, and J. U. Raj Long-term effects of prenatal hypoxia on endothelium-dependent relaxation responses in pulmonary arteries of adult sheep Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L547 - L554. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. G. da Silva, A. Specht, B. Wegiel, C. Ferran, and E. Kaczmarek Mechanism of Purinergic Activation of Endothelial Nitric Oxide Synthase in Endothelial Cells Circulation, February 17, 2009; 119(6): 871 - 879. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. M. Piper, D. Garcia-Dorado, and E. A. Martinson Monitoring the success of articles: a look back and a look ahead Cardiovasc Res, December 1, 2008; 80(3): 321 - 323. [Full Text] [PDF] |
||||
![]() |
C. R. Schnell, F. Stauffer, P. R. Allegrini, T. O'Reilly, P. M.J. McSheehy, C. Dartois, M. Stumm, R. Cozens, A. Littlewood-Evans, C. Garcia-Echeverria, et al. Effects of the Dual Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Inhibitor NVP-BEZ235 on the Tumor Vasculature: Implications for Clinical Imaging Cancer Res., August 15, 2008; 68(16): 6598 - 6607. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Ibiza, A. Perez-Rodriguez, A. Ortega, A. Martinez-Ruiz, O. Barreiro, C. A. Garcia-Dominguez, V. M. Victor, J. V. Esplugues, J. M. Rojas, F. Sanchez-Madrid, et al. Endothelial nitric oxide synthase regulates N-Ras activation on the Golgi complex of antigen-stimulated T cells PNAS, July 29, 2008; 105(30): 10507 - 10512. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Zeng and Q. Xu eNOS-ER{alpha} Complex Goes to Telomerase Circ. Res., July 3, 2008; 103(1): 10 - 12. [Full Text] [PDF] |
||||
![]() |
C. I. Jones III, Z. Han, T. Presley, S. Varadharaj, J. L. Zweier, G. Ilangovan, and B. R. Alevriadou Endothelial cell respiration is affected by the oxygen tension during shear exposure: role of mitochondrial peroxynitrite Am J Physiol Cell Physiol, July 1, 2008; 295(1): C180 - C191. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. L. Kellogg Jr., J. L. Zhao, and Y. Wu Endothelial nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H123 - H129. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. N. White, E. J. Hamilton, A. Garcia, D. Wang, K. K. M. Chia, G. A. Figtree, and H. H. Rasmussen Opposing effects of coupled and uncoupled NOS activity on the Na+-K+ pump in cardiac myocytes Am J Physiol Cell Physiol, February 1, 2008; 294(2): C572 - C578. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Lamas, C. J. Lowenstein, and T. Michel Nitric oxide signaling comes of age: 20 years and thriving Cardiovasc Res, July 15, 2007; 75(2): 207 - 209. [Full Text] [PDF] |
||||










