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Cardiovascular Research 2007 74(1):159-168; doi:10.1016/j.cardiores.2007.01.012
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Copyright © 2007, European Society of Cardiology

Syk contributes to PDGF-BB-mediated migration of rat aortic smooth muscle cells via MAPK pathways

Chang-Kwon Leea,1, Hwan Myung Leea,1, Hyo Jin Kima, Hyo-Jun Parka, Kyung-Jong Wona, Hui Yul Roha, Wahn Soo Choia, Byeong Hwa Jeonc, Tae-Kyu Parkb and Bokyung Kima,*

aDepartments of Physiology and Immunology, College of Medicine, Konkuk University, Danwol-dong 322, Chungju 380-701, Republic of Korea
bDivision of Life Science, Konkuk University, Danwol-dong 322, Chungju 380-701, Republic of Korea
cDepartment of Physiology, Chungnam National University, Taejeon 301-131, Republic of Korea

* Corresponding author. Tel.: +82 43 8403726; fax: +82 43 8519329. Email address: bkkim2{at}kku.ac.kr

Objective: Here we investigated the role of spleen tyrosine kinase (Syk) in the migration induced by platelet-derived growth factor (PDGF) in rat aortic smooth muscle cells (RASMC).

Methods: Cell migration was determined using a Boyden chamber, by wound-healing, and by aortic ring assays. Activity of Syk, mitogen-activated protein kinase (MAPK), and heat shock protein 27 (HSP27) were tested using immunoblotting with kinase inhibitors and small interference RNAs.

Results: PDGF-BB induced binding of Syk to the PDGFβ receptor and increased the phosphorylation of Syk and migration in RASMC. These effects of PDGF-BB were inhibited by piceatannol, an inhibitor of Syk. PDGF-BB increased the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, p38 MAPK, and HSP27, which were significantly inhibited by piceatannol and in Syk-knockdown cells. The p38 MAPK inhibitor SB203580 and ERK1/2 inhibitor PD98059 inhibited the migration, which was further inhibited by the combination of these inhibitors. SB203580, but not PD98059, inhibited the phosphorylation of HSP27 induced by PDGF-BB in RASMC. PDGF-BB-induced migration was attenuated in HSP27-knockdown cells. Kinase inhibitors and Syk-knockdown diminished PDGF-BB-induced sprout outgrowth in the aortic ring assay.

Conclusions: These results imply that Syk is an upstream signal of the p38 MAPK/HSP27 and ERK1/2 pathways that contributes to PDGF-BB-mediated migration in RASMC.

KEYWORDS Smooth muscle; Growth factor; MAP kinase; Tyrosine protein kinase; Remodeling

1 Contributed equally to the manuscript.

Time for primary review 34 days


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