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Cardiovascular Research 2007 73(3):587-596; doi:10.1016/j.cardiores.2006.11.018
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Copyright © 2006, European Society of Cardiology

The nitroxyl anion (HNO) is a potent dilator of rat coronary vasculature

Joanne L. Favaloro* and Barbara K. Kemp-Harper

Department of Pharmacology and Centre for Vascular Health Initiative, School of Biomedical Sciences, Monash University, Clayton Vic 3800, Australia

* Corresponding author. Tel.: +61 3 9905 4674; fax: +61 3 9905 5851. Email address: joanne.favaloro{at}med.monash.edu.au

Objective: The nitroxyl anion (HNO) is the one-electron reduction product of NO·. This redox variant has been shown to be endogenously produced and to have effects that are pharmacologically distinct from NO·. This study investigates the vasodilator and chronotropic effects of HNO in the rat isolated coronary vasculature.

Methods: Sprague-Dawley rat hearts were retrogradely perfused with Krebs' solution (8 ml/min) using the Langendorff technique. Perfusion pressure was raised using a combination of infusion of phenylephrine and bolus additions of the thromboxane mimetic U46619 [GenBank] to attain a baseline perfusion pressure of 100–120 mm Hg. The vasodilator effects of a nitroxyl anion donor, Angeli's salt, were examined in the absence and presence of HNO and NO· scavengers, K+ channel inhibition, and soluble guanylate cyclase (sGC) inhibition. In addition, the inotropic and chronotropic effects of Angeli's salt were examined in hearts at resting perfusion pressure (50–60 mm Hg) and compared to responses evoked by acetylcholine and isoprenaline.

Results: Angeli's salt causes a potent and reproducible vasodilatation in isolated perfused rat hearts. This response is unaffected by the NO· scavenger hydroxocobalamin (0.1 mM) but is significantly inhibited by the HNO scavenger N-acetyl-L-cysteine (4 mM), suggesting that HNO is the mediator of the observed responses. Vasodilatation responses to Angeli's salt were virtually abolished in the presence of the sGC inhibitor ODQ (10 µM). The magnitude of the vasodilatation response to Angeli's salt was significantly reduced in the presence of 30 mM K+, 10 µM glibenclamide and in the presence of the calcitonin gene-related peptide (CGRP) antagonist CGRP(8–37) (0.1 µM). Angeli's salt had little effect on heart rate or force of contraction, whilst isoprenaline and acetylcholine elicited significant positive and negative cardiotropic effects, respectively.

Conclusions: The HNO donor Angeli's salt elicits a potent and reproducible vasodilatation response. The results suggest that the response is elicited by HNO through sGC-mediated CGRP release and KATP channel activation.

KEYWORDS Nitroxyl anion; Vasoactive agents; Coronary circulation; Nitric oxide; Redox signaling

Time for primary review 33 days


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