Age-dependent changes in myocardial matrix metalloproteinase/tissue inhibitor of metalloproteinase profiles and fibroblast function

doi:10.1016/j.cardiores.2004.11.029

Cardiovascular Research 2005 66(2):410-419; doi:10.1016/j.cardiores.2004.11.029

This Article

	Full Text
	FREE Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Add to My Personal Archive
	Download to citation manager
	Request Permissions

Google Scholar

	Articles by Lindsey, M. L.
	Articles by Spinale, F. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lindsey, M. L.
	Articles by Spinale, F. G.

Social Bookmarking

	What's this?

Age-dependent changes in myocardial matrix metalloproteinase/tissue inhibitor of metalloproteinase profiles and fibroblast function

Merry L. Lindsey^a^,*, Danielle K. Goshorn^a, Christina E. Squires^a, G. Patricia Escobar^a, Jennifer W. Hendrick^a, Joseph T. Mingoia^a, Sarah E. Sweterlitsch^a and Francis G. Spinale^a^,b

^aDivision of Cardiothoracic Surgery Research, Room 629, Strom Thurmond Research Building, 770 MUSC Complex, Medical University of South Carolina, 114 Doughty Street, P.O. Box 250778, Charleston, SC 29425, United States
^bRalph A. Johnson Veterans Administration Medical Center, Charleston, SC 29425, United States

^* Corresponding author. Tel.: +-1 843 876 5186; fax: +-1 843 876 5187. Email address: lindseml{at}musc.edu

Objective: To evaluate the effects of aging on left ventricular(LV) geometry, collagen levels, matrix metalloproteinase (MMP)and tissue inhibitor of metalloproteinase (TIMP) abundance,and myocardial fibroblast function.

Methods: Young (3-month-old; n=28), middle-aged (MA; 15-month-old;n=17), and old (23-month-old; n=16) CB6F1 mice of both sexeswere used in this study. Echocardiographic parameters were measured;collagen, MMP, and TIMP levels were determined for both thesoluble and insoluble protein fractions; and fibroblast functionwas evaluated.

Results: LV end-diastolic dimensions and wall thickness increasedin both MA and old mice, accompanied by increased soluble proteinand decreased insoluble collagen. Immunoblotting revealed differentialMMP/TIMP profiles. Compared to MA levels, MMP-3, MMP-8, MMP-9,MMP-12, and MMP-14 increased, and TIMP-3 and TIMP-4 decreasedin the insoluble fraction of old mice, suggesting increasedextracellular matrix (ECM) degradative capacity. Fibroblastproliferation was blunted with age.

Conclusion: This study, for the first time, identified specificdifferences in cellular and extracellular processes that likelycontribute to age-dependent ECM remodeling.

KEYWORDS Matrix metalloproteinase; Tissue inhibitor of metalloproteinase; Extracellular matrix; Matrix remodeling

Time for primary review 28 days

Add to CiteULike CiteULike Add to Connotea Connotea Add to Del.icio.us Del.icio.us What's this?

This article has been cited by other articles:

Y. Yang, Y. Ma, W. Han, J. Li, Y. Xiang, F. Liu, X. Ma, J. Zhang, Z. Fu, Y.-D. Su, et al.
Age-related differences in postinfarct left ventricular rupture and remodeling
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1815 - H1822.
[Abstract] [Full Text] [PDF]

M. Leslie
Environmental movement.
Sci. Aging Knowl. Environ., March 15, 2006; 2006(7): nf9 - nf9.
[Abstract] [Full Text]

S. Pepe and E. G. Lakatta
Aging hearts and vessels: Masters of adaptation and survival
Cardiovasc Res, May 1, 2005; 66(2): 190 - 193.
[Full Text] [PDF]

				M. Leslie Environmental movement. Sci. Aging Knowl. Environ., March 15, 2006; 2006(7): nf9 - nf9. [Abstract] [Full Text]

				S. Pepe and E. G. Lakatta Aging hearts and vessels: Masters of adaptation and survival Cardiovasc Res, May 1, 2005; 66(2): 190 - 193. [Full Text] [PDF]