Copyright © 2004, European Society of Cardiology
Modulatory role of the adventitia on noradrenaline and angiotensin II responses
Role of endothelium and AT2 receptors
aDepartamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain
bDepartamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain
* Corresponding author. Unidad de Cartografía Cerebral, Instituto Pluridisciplinar, Paseo Juan XXIII, no. 1, Madrid 28040, Spain. Tel.: +34 91 394 3261; fax: +34 91 394 3265. Email address: marisolf{at}farm.ucm.es
Objective: We have studied the modulatory role of the adventitia on vascular tone and nitric oxide (NO) availability in response to noradrenaline (NA) and angiotensin II (Ang II).
Methods: Changes in isometric tension were determined in carotid arteries from 3-month-old Sprague–Dawley rats denuded from adventitia (–A) and compared to intact rings (+A). NO availability was assessed by the fluorescent NO indicator, 4,5-diaminofluorescein diacetate (DAF-2).
Results: Responses to NA (10–10 to 10–6 M) were: (i) significantly lower in –A compared to +A rings; (ii) equally enhanced in +A and –A rings without endothelium; and (iii) reduced in +A and –A rings incubated with superoxide dismutase (SOD; 15 U/ml). Responses to Ang II (10–10 to 10–7 M) were: (i) similar between +A and –A segments; (ii) equally reduced in both groups by SOD; and (iii) increased by endothelial denudation in both +A and –A arteries. Blockade of AT2 receptors with PD 123,319 (10–7 M) significantly increased Ang II-induced contractions in +A rings. In segments preincubated with losartan (10–5 M) and precontracted with NA (10–7 M), Ang II elicited a relaxation that was abolished by L-NAME (10–4 M), PD 123,319 (10–7 M), and endothelium or adventitial removal. NO availability was increased in carotid rings stimulated with Ang II, but not with NA. This NO release was blocked by PD 123,319 (10–7 M) and endothelium denudation.
Conclusions: These results suggest that the adventitia differently modulates responses to vasoconstrictors and that it is a key layer in Ang II-induced contractions, mediating NO release from the endothelium via AT2 receptors. This increase in NO counterbalances basal superoxide release.
KEYWORDS Adventitia; Angiotensin II; AT2 receptors; Noradrenaline; Nitric oxide
Time for primary review 40 days
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