Skip Navigation

Cardiovascular Research 2005 65(1):254-262; doi:10.1016/j.cardiores.2004.09.010
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lauer, N.
Right arrow Articles by Kojda, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lauer, N.
Right arrow Articles by Kojda, G.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2004, European Society of Cardiology

Critical involvement of hydrogen peroxide in exercise-induced up-regulation of endothelial NO synthase

Nadine Lauera, Tatsiana Suvoravaa, Ulrich Rütherb, Ralf Jacobc, Wilfried Meyerd, David G. Harrisone and Georg Kojdaa,*

aInstitut für Pharmakologie und Klinische Pharmakologie, Heinrich-Heine-Universität, Düsseldorf, Germany http://www.kojda.de
bInstitut für Entwicklungsbiologie der Tiere, Heinrich-Heine-Universität, Düsseldorf, Germany
cInstitut für Zellbiologie und Zellpathologie, Philipps-Universität, Marburg Germany
dAnatomisches Institut, Tierärztliche Hochschule, Hanover, Germany
eCardiology Division, Emory University, Atlanta, GA, USA

* Corresponding author. Institut für Pharmakologie und Klinische Pharmakologie, Heinrich-Heine-Universität, Moorenstr. 5, 40225 Düsseldorf, Germany. Tel.: +49 211 81 12518; fax: +49 211 81 14781. Email address: kojda{at}uni-duesseldorf.de

Objective: Recent studies from our groups have indicated that endothelial nitric oxide synthase (eNOS) expression is increased in cell culture by both shear stress and by hydrogen peroxide (H2O2). In vivo, exercise training, known to increase both endothelial shear stress and oxidative stress, also increases eNOS expression. It is unclear if H2O2 contributes to an increase in eNOS expression in response to exercise training.

Methods: To address this question, we generated mice overexpressing human catalase (hCat) driven by the murine Tie-2 promoter to specifically target this transgene to the endothelium (cat++).

Results: Vessels of cat++ expressed significantly higher levels of catalase mRNA and catalase protein and activity but normal levels of eNOS. Exercise alone had no effect on catalase expression in C57BL/6. Wild-type littermates of cat++ showed an increase in eNOS expression with 3 weeks of exercise (2.53 ± 0.42-fold) comparable to C57BL/6 (2.93 ± 0.45-fold). In striking contrast, 3 weeks of exercise had no effect on aortic (1.33 ± 0.32-fold) and myocardial (1.1 ± 0.2-fold) eNOS expression in catalase transgenic mice.

Conclusions: These data suggest that endogenous H2O2 plays a key role in the endothelial adaptation to exercise training by stimulating an up-regulation of eNOS.

KEYWORDS Oxidative stress; Hydrogen peroxide; Exercise; Endothelial nitric oxide synthase; Catalase; Vascular endothelial function


Time for primary review 19 days


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
J. Physiol.Home page
Q.-J. Zhang, S. L. McMillin, J. M. Tanner, M. Palionyte, E. D. Abel, and J. D. Symons
Endothelial nitric oxide synthase phosphorylation in treadmill-running mice: role of vascular signalling kinases
J. Physiol., August 1, 2009; 587(15): 3911 - 3920.
[Abstract] [Full Text] [PDF]


Home page
Physiol. Rev.Home page
J.-L. Balligand, O. Feron, and C. Dessy
eNOS Activation by Physical Forces: From Short-Term Regulation of Contraction to Chronic Remodeling of Cardiovascular Tissues
Physiol Rev, April 1, 2009; 89(2): 481 - 534.
[Abstract] [Full Text] [PDF]


Home page
J. Appl. Physiol.Home page
E. M. F. Van Craenenbroeck, C. J. Vrints, S. E. Haine, K. Vermeulen, I. Goovaerts, V. F. I. Van Tendeloo, V. Y. Hoymans, and V. M. A. Conraads
A maximal exercise bout increases the number of circulating CD34+/KDR+ endothelial progenitor cells in healthy subjects. Relation with lipid profile
J Appl Physiol, April 1, 2008; 104(4): 1006 - 1013.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Akita, H. Otani, S. Matsuhisa, S. Kyoi, C. Enoki, R. Hattori, H. Imamura, H. Kamihata, Y. Kimura, and T. Iwasaka
Exercise-induced activation of cardiac sympathetic nerve triggers cardioprotection via redox-sensitive activation of eNOS and upregulation of iNOS
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2051 - H2059.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
N. L. Reynaert, S. W. Aesif, T. McGovern, A. Brown, E. F. M. Wouters, C. G. Irvin, and Y. M. W. Janssen-Heininger
Catalase Overexpression Fails to Attenuate Allergic Airways Disease in the Mouse
J. Immunol., March 15, 2007; 178(6): 3814 - 3821.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
M. Ushio-Fukai
Redox signaling in angiogenesis: Role of NADPH oxidase
Cardiovasc Res, July 15, 2006; 71(2): 226 - 235.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
S. Lehoux
Redox signalling in vascular responses to shear and stretch
Cardiovasc Res, July 15, 2006; 71(2): 269 - 279.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. P. De Bono, D. Adlam, D. J. Paterson, and K. M. Channon
Novel quantitative phenotypes of exercise training in mouse models
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R926 - R934.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
W. Cao, C. Fiocchi, and V. E. Pricolo
Production of IL-1{beta}, hydrogen peroxide, and nitric oxide by colonic mucosa decreases sigmoid smooth muscle contractility in ulcerative colitis
Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1408 - C1416.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
T. Suvorava, N. Lauer, S. Kumpf, R. Jacob, W. Meyer, and G. Kojda
Endogenous Vascular Hydrogen Peroxide Regulates Arteriolar Tension In Vivo
Circulation, October 18, 2005; 112(16): 2487 - 2495.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
D. D. Gutterman
Mitochondria and Reactive Oxygen Species: An Evolution in Function
Circ. Res., August 19, 2005; 97(4): 302 - 304.
[Full Text] [PDF]


Home page
Cardiovasc ResHome page
G. Kojda and R. Hambrecht
Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy?
Cardiovasc Res, August 1, 2005; 67(2): 187 - 197.
[Abstract] [Full Text] [PDF]



Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.