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Cardiovascular Research 2005 65(1):203-210; doi:10.1016/j.cardiores.2004.09.001
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Copyright © 2004, European Society of Cardiology

Heart failure increases protein expression and enzymatic activity of heme oxygenase-1 in the lung

Chen F. Lama,b, Anthony J. Croattc, Darcy M. Richardsona, Karl A. Nathc and Zvonimir S. Katusica,b,*

aDepartments of Anesthesiology, Mayo Clinic and Foundation, Rochester, MN, USA
bClinical Pharmacology and Therapeutics, Mayo Clinic and Foundation, Rochester, MN, USA
cDivision of Nephrology, Mayo Clinic and Foundation, Rochester, MN, USA

* Corresponding author. Department of Anesthesiology, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA. Tel.: +1 507 255 4288; fax: +1 507 255 7300. Email address: katusic.zvonimir{at}mayo.edu

Objective: Heart failure (HF) cell or siderophages are pulmonary macrophages that phagonicytize erythrocytes leaked from the congested capillaries due to HF. Degradation of erythrocytes and hemoglobin increases concentrations of heme in the lung. We hypothesized that the HF-induced increase in the concentration of heme up-regulates the expression and enzymatic activity of heme oxygenase (HO)-1 in the lung.

Methods: Using the aortocaval (AC) fistula model of HF, we examined the following parameters 8–10 weeks after the creation of the fistula: morphological changes in the lung by Prussian blue iron and immunohistochemical staining, HO-1 protein expression and activity in the rat lungs, and concentrations of nitrite/nitrate (NOx–) and cyclic guanosine 3',5'-monophospate (cGMP) of the lung homogenates.

Results: Iron-stained siderophages were observed only in the lungs of rats with AC fistula. Protein level and enzyme activity of HO-1 were significantly enhanced in the lung of HF rats. NOx– concentrations of the two groups were similar, but cGMP was elevated in the lung of AC fistula rats (0.34 ± 0.06 vs. 0.89 ± 0.20 pmol/mg protein, P=0.025). Staining of serial sections of the lung tissues demonstrated induction of HO-1 co-localized to iron-stained siderophages.

Conclusions: HF causes increased pulmonary HO-1 expression and activity, which emanates largely from siderophages. Up-regulation of HO-1 may have pulmonary protective in HF.

KEYWORDS Heart failure; Siderophages; Heme, oxygenase-1; p38 MAPK; cGMP

Time for primary review 17 days


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