© 2004 by European Society of Cardiology
Copyright © 2004, European Society of Cardiology
Adjunctive 17β-estradiol administration reduces infarct size by altered expression of canine myocardial connexin43 protein
aCardiology Section, Departments of Internal Medicine, Taipei Medical University and Chi-Mei Medical Center, Tainan, Taiwan
bNational Taiwan University and Department of Pharmacy, National Taiwan University Hospital, Taipei, Taiwan
cDepartment of Surgery, Municipal Jen-Ai Hospital, Taipei, Taiwan
dCardiology Section, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
eDepartment of Medicine, Cardiology Section, Taipei Medical University and Hospital, Taipei, Taiwan
*Corresponding authors. Dr. Tsung-Ming Lee is to be contacted at Cardiology Section, Department of Internal Medicine, Taipei Medical University and Chi-Mei Medical Center, 901, Chung-Hwa Road, Yang-Kan City, Tainan 710, Taiwan. Tel.: +886-6-281-2811x2663; fax: +886-6-283-2639. Dr. Nen-Chung Chang, Cardiology Section, Department of Medicine, Taipei Medical University and Hospital, 252, Wu-Hsing St., Taipei, Taiwan. Tel.: +886-2-27372181x3101; fax: +886-2-23911200. Email address: tsungm.lee{at}msa.hinet.net ncchang{at}tmu.edu.tw
Background: Traffic of potentially harmful cytosolic messengers through gap junctions might cause increased injury during ischemia. The present study was to determine whether the infarct size-reducing effect of adjunctive estradiol administration prior to reperfusion is associated with an attenuated expression of connexin43 at the border of infarction in a canine model. Methods: Experiments were performed in 48 dogs (n=16 each group), assigned to receive either vehicle (control group), 17β-estradiol administered before coronary occlusion (early group), or 3 min before coronary reperfusion following 60-min ischemia (late group). Changes in the amount of phosphorylated connexin43 were measured by Western blot. Results: Infarct size was significantly larger in the control (38±7% of area at risk) than in the supplemented groups (16±6% in the early group; 16±8% in the late group, P<0.0001, both). Reperfusion caused a significant elevation in free radicals as measured by lucigenin-derived chemiluminescence. The rise of free radicals was significantly inhibited in animals treated with estrogen, either early or late. The amount of phosphorylated connexin43 was reduced, as assessed by Western blot in control hearts at the border zone. These changes were significantly enhanced by estrogen administration. The magnitude of infarct size positively correlated with the magnitude of phosphorylated connexin43 expression assessed by Western blot (r=0.83, P<0.0001). Confocal microscopy confirmed the changes of junctional complexes. Conclusions: This result demonstrated that the cardioprotective effect of estrogen as an antioxidant may be associated with the reduced amount of phosphorylated myocardial connexin43 protein.
KEYWORDS Connexin43; Contraction band necrosis; Estradiol; Myocardial infarction
Time for primary review 25 days
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