© 2003 by European Society of Cardiology
Copyright © 2003, European Society of Cardiology
Shear stress-induced up-regulation of the intermediate-conductance Ca2+-activated K+ channel in human endothelium
aDepartment of Nephrology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, D-12200 Berlin, Germany
bDepartment of Physiology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Arnimallee 22, D-14195 Berlin, Germany
cDepartment of Gynecology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, D-12200 Berlin, Germany
*Corresponding author. Tel.: +49-30-84452398; fax: +49-30-84452398. Email address: koe{at}zedat.fu-berlin.de
Objective: Wall shear stress associated with blood flow is a major stimuli for generation of endothelial vasodilating and antithrombotic factors and it also regulates endothelial gene expression. Activation of endothelial intermediate-conductance Ca2+-activated K+ channels (IKCa) is important for the control of endothelial function by inducing cell hyperpolarization and thus generation of the endothelium-derived hyperpolarizing factor. In the present study we tested whether the IKCa encoding IKCa1 gene is regulated by laminar shear stress (LSS). Methods: Human umbilical vein endothelial cells (HUVEC) were subjected to LSS with a magnitude of 0.5–15 dyn/cm2 and time intervals of 2–24 h in a flow cone apparatus. Expression of the IKCa1 gene and IKCa-functions were determined by using real time RT-PCR and patch-clamp techniques. Results: A short 2–4 h—or long 24 h—exposure to a LSS with a low (venous) magnitude of 0.5 dyn/cm2 had no effect on IKCa1 expression levels. An exposure for 2 and 4 h to LSS with an intermediate magnitude of 5 dyn/cm2 was also ineffective, whereas an exposure for 24 h induced a significant threefold up-regulation of IKCa1 expression levels. An exposure to LSS with a higher (arterial) magnitude of 15 dyn/cm2, resulted in an eightfold up-regulation of IKCa1 expression levels after a 4 h—exposure and a fourfold increase of IKCa1 expression levels at 24 h. The increased IKCa1 expression levels following exposure to high levels of LSS resulted in enhanced IKCa whole-cell currents and in an increased hyperpolarization of the endothelium in response to ATP and the IKCa opener 1-EBIO. Inhibition of the mitogen-activated protein kinase/extracellular-signal-regulated kinase (ERK) kinase 1/2 (MEK/ERK) pathway by PD98059 prevented the LSS-induced up-regulation of IKCa1 expression levels and IKCa whole-cell currents indicating that augmentation of IKCa1 expression levels is mediated by the LSS-induced activation of the MEK/ERK pathway. Conclusion: Long term exposure to LSS up-regulates expression and function of endothelial IKCa. This increase might represent a new important mechanism in endothelial adaptation to altered hemodynamics.
KEYWORDS Blood flow; Endothelial function; Signal transduction; Gene expression; IKCa1; K-channel
Time for primary review 29 days
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