© 2003 by European Society of Cardiology
Copyright © 2003, European Society of Cardiology
Angiotensin II type 1 receptor participates in extracellular matrix production in the late stage of remodeling after vascular injury
The First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan
* Corresponding author. Tel.: +81-99-275-5318; fax: +81-99-275-5322. biro{at}m2.kufm.kagoshima-u.ac.jp
Objective: Extracellular matrix (ECM) accumulation is important in restenosis after angioplasty. Underlying molecular mechanisms remain to be elucidated, especially in vivo. We investigated expression of angiotensin II type 1 receptor (ATR1) in a rat model for up to 24 weeks after vascular injury, and also the effect of an ATR1 antagonist on neointimal thickening and ECM production. Methods and Results: Carotid arteries of rats were injured with a balloon catheter and then removed at 2, 5, and 7 days and 2, 4, 8, 16, and 24 weeks after injury. Although ATR1 immunoreactivity was slightly detectable in smooth muscle cells (SMC) in the media of uninjured arteries, reactivity was strong in neointimal SMC even 24 weeks after injury. Western blotting demonstrated similar results. ATR1 mRNA also was upregulated in neointimal SMC even 24 weeks after injury, as indicated by RT-PCR and by in situ hybridization. Candesartan, an ATR1 antagonist, significantly inhibited histologically evident neointimal thickening and collagen and elastin accumulation at 8 weeks after injury whether given beginning 1 day before injury, 4 days after injury, or 7 days after injury. Conclusion: ATR1 is upregulated in the late stage of remodeling after vascular injury and is important in ECM production.
KEYWORDS Angiotensin; Extracellular matrix; Receptors; Remodeling; Smooth muscle
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
S. Lacchini, A. S. Heimann, F. S. Evangelista, L. Cardoso, G. J. J. Silva, and J. E. Krieger Cuff-induced vascular intima thickening is influenced by titration of the Ace gene in mice Physiol Genomics, May 13, 2009; 37(3): 225 - 230. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Heeneman, J. C. Sluimer, and M. J.A.P. Daemen Angiotensin-Converting Enzyme and Vascular Remodeling Circ. Res., August 31, 2007; 101(5): 441 - 454. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Li, C. Zhang, S. Schaefer, A. Estes, and K. U. Malik ANG II-induced neointimal growth is mediated via cPLA2- and PLD2-activated Akt in balloon-injured rat carotid artery Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2592 - H2601. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Forrest and C. McNamara Id Family of Transcription Factors and Vascular Lesion Formation Arterioscler Thromb Vasc Biol, November 1, 2004; 24(11): 2014 - 2020. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. L. Schiffrin and R. M. Touyz From bedside to bench to bedside: role of renin-angiotensin-aldosterone system in remodeling of resistance arteries in hypertension Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H435 - H446. [Full Text] [PDF] |
||||



