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Cardiovascular Research 2003 58(2):444-450; doi:10.1016/S0008-6363(02)00834-9
© 2003 by European Society of Cardiology
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Copyright © 2003, European Society of Cardiology

Endoventricular porcine autologous myoblast transplantation can be successfully achieved with minor mechanical cell damage

Bénédicte Chazauda, Luc Hittingerb,c,d, Corinne Sonneta, Stéphane Champagneb, Philippe Le Corvoisierc, Nicole Benhaiem-Sigauxe, Thierry Unterseehf, Jinbo Suc, Pascal Merletg, Alain Rahmounih, Jérôme Garotb,c, Romain Gherardia,e and Emmanuel Teigerf,i,*

aINSERM E0011, Faculté de Médecine, Créteil, France
bFédération de Cardiologie, Hôpital Henri Mondor, Créteil, France
cINSERM U400, Faculté de Médecine, Créteil, France
dCentre de Recherche Chirurgicales, Hôpital Henri Mondor, Créteil, France
eService d’Histo-embryologie-cytogénétique, Hôpital Henri Mondor, Créteil, France
fService des Explorations Fonctionnelles, Hôpital Henri Mondor, 51 Avenue du Maréchal DeLattre de Tassigny, 94010 Créteil Cedex, France
gService de Médecine Nucléaire, Hôpital Henri Mondor, Créteil, France
hService de Radiologie et Imagerie Médicale, Hôpital Henri Mondor, Créteil, France
iINSERM U492, Faculté de Médecine, Créteil, France

teiger{at}creteil.inserm.fr

* Corresponding author. Tel.: +33-1-4981-2677; fax: +33-1-4981-2667.

Objective: Transplantation of skeletal myogenic precursor cells (mpc) into the myocardium using a non-surgical procedure. Methods: Closed-chest mpc transplantation was assessed in pigs using the NOGA-Biosense® device allowing both electromechanical mapping of the left ventricle (LV), and guided mpc injections through endocardium. Results: We successively established that: (1) adequate preimplantation handling of mpc can be achieved when mpc are kept in 0.1% serum albumin-containing medium until implantation; (2) mpc are neither retained nor destroyed in the catheter or the needle and their passage does not affect their survival, growth and differentiation; (3) large numbers of autologous mpc can be actually transplanted in the LV myocardium by transendocardial route, as assessed by post-mortem examination of pigs injected with iron-loaded mpc; (4) cell injection into the myocardium does not induce conspicuous cell mortality since more than 80% of mpc recovered from LV tissue are alive 15 min after injection; (5) mpc injections can be guided into circumscribed LV targets such as infarcted areas, as assessed by comparison of map injection sites with location of iron-loaded mpc at post-mortem examination of LV myocardium. Conclusion: This new approach may pave the way for a large spectrum of cell therapies targeting myocardial diseases.

KEYWORDS Heart failure; Infarction; Myocytes; Stem cells; Transplantation


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