Skip Navigation

Cardiovascular Research 2003 57(1):186-194; doi:10.1016/S0008-6363(02)00655-7
© 2003 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wang, D.
Right arrow Articles by Jankowski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, D.
Right arrow Articles by Jankowski, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2003, European Society of Cardiology

Genistein supplementation stimulates the oxytocin system in the aorta of ovariectomized rats

Donghao Wanga, Jolanta Gutkowskaa,c, Mieczyslaw Marcinkiewiczb, Grazyna Rachelskaa and Marek Jankowskia,c,*

aLaboratory of Cardiovascular Biochemistry, Centre Hospitalier de l’Université de Montréal–Hôtel-Dieu, 3850 St. Urbain Street, Masson Pavilion, Montreal, Quebec, Canada H2W 1T7
bCytochem, Montreal, Quebec, Canada
cDepartment of Medicine, Université de Montréal, Montreal, Quebec, Canada

* Corresponding author. Tel.: +1-514-890-8000x12757; fax: +1-514-412-7199. marek.jankowski{at}umontreal.ca

Objective: In the present study, we localized oxytocin (OT) and its receptor (OTR) in the rat aorta, and investigated whether genistein, an isoflavonic phytoestrogen, influences their expression in ovariectomized (OVX) rats deficient in estrogen. Methods and results: OVX Sprague–Dawley rats were randomized to the following groups: genistein (from 0.02 to 5 µg/g/day, s.c. for 10 days), estradiol (E2, 0.1 µg/g/day, s.c. for 10 days) or their respective vehicles. OT and OTR immunostaining was concentrated in the aortic tunica intima, suggesting their paracrine/autocrine action within endothelial cells. Reverse transcription-polymerase chain reaction analysis showed that 1 and 5 µg/g but not 0.1 µg/g genistein elevated OT mRNA (2-fold P<0.05), OTR mRNA (2.5-fold, P<0.05) and endothelial nitric oxide synthase (eNOS) mRNA (2-fold, P<0.05) in the aorta of OVX rats. In addition, genistein treatment increased estrogen receptor {alpha} (ER{alpha}) (2- to 3-fold, P<0.05) but resulted in a 50% decrease of ERβ (P<0.05). These genistein effects were neutralized by treatment of OVX rats with the ER antagonist ICI 182,780 (1.5 µg/g/day, s.c. for 10 days). Similarly, Western blot analysis revealed an increase of 67-kDa OTR, 140-kDa eNOS, 62-kDa ER{alpha} and a decrease of 55-kDa ERβ (P<0.05) in the aorta of OVX rats treated with genistein. In contrast, the treatment of OVX rats with E2 elevated ERβ mRNA (1.5 fold, P<0.05) but similarly to genistein increased OT, OTR, eNOS and ER{alpha} mRNA. Conclusion: These results provide the first evidence of OT and OTR co-localization in endothelial cells. The response to genistein via ER activation can be regarded as a recovery from endothelial dysfunction induced by ovariectomy.

KEYWORDS Endothelial function; Gene expression; Hormones; Receptors; Vasoconstriction/dilation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Gutkowska, M. Miszkurka, B. Danalache, N. Gassanov, D. Wang, and M. Jankowski
Functional arginine vasopressin system in early heart maturation
Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2262 - H2270.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Gutkowska, A. Paquette, D. Wang, J.-M. Lavoie, and M. Jankowski
Effect of exercise training on cardiac oxytocin and natriuretic peptide systems in ovariectomized rats
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R267 - R275.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
M Jankowski, D Wang, S Mukaddam-Daher, and J Gutkowska
Pregnancy alters nitric oxide synthase and natriuretic peptide systems in the rat left ventricle
J. Endocrinol., January 1, 2005; 184(1): 209 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
G.-S. Lee, K.-C. Choi, H.-J. Kim, and E.-B. Jeung
Effect of Genistein As a Selective Estrogen Receptor Beta Agonist on the Expression of Calbindin-D9k in the Uterus of Immature Rats
Toxicol. Sci., December 1, 2004; 82(2): 451 - 457.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Jankowski, B. Danalache, D. Wang, P. Bhat, F. Hajjar, M. Marcinkiewicz, J. Paquin, S. M. McCann, and J. Gutkowska
Oxytocin in cardiac ontogeny
PNAS, August 31, 2004; 101(35): 13074 - 13079.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Vignozzi, S. Filippi, M. Luconi, A. Morelli, R. Mancina, M. Marini, G. B. Vannelli, S. Granchi, C. Orlando, S. Gelmini, et al.
Oxytocin Receptor Is Expressed in the Penis and Mediates an Estrogen-Dependent Smooth Muscle Contractility
Endocrinology, April 1, 2004; 145(4): 1823 - 1834.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.