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Cardiovascular Research 2002 55(1):161-170; doi:10.1016/S0008-6363(02)00329-2
© 2002 by European Society of Cardiology
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Copyright © 2002, European Society of Cardiology

Coronary function and adenosine receptor-mediated responses in ischemic-reperfused mouse heart

Amanda J Flood, Laura Willems and John P Headrick*

Heart Foundation Research Centre, School of Health Science, Griffith University Gold Coast Campus, Southport, Qld 4217, Australia

* Corresponding author. Tel.: +61-7-5552-8292; fax: +61-7-5552-8802 j.headrick{at}mailbox.gu.edu.au

Objectives: To assess the impact of ischemia-reperfusion (I/R) on coronary function, and the role of endogenous adenosine in modifying post-ischemic vascular function in asanguinous hearts. Methods: Vascular function was studied in Langendorff perfused mouse hearts subjected to 20–25-min ischemia and 30-min reperfusion. Results: Ischemia altered the dependence of flow on work-rate observed in normoxic hearts, and inhibited reflow by mechanisms additional to diastolic compression. Coronary responses were selectively reduced: 2-chloroadenosine and ADP dilated with pEC50s of 8.4±0.1 and 7.4±0.1 in non-ischemic hearts versus 7.7±0.1 and 7.1±0.1 after 20-min ischemia (P<0.05). Sensitivity was further reduced after 25-min ischemia. Responses to nitroprusside were unaltered. NO-synthase antagonism (50 µM nitro-L-arginine methylester) reduced sensitivities to 2-chloroadenosine and ADP up to 10-fold, and eliminated inhibitory effects of I/R. KATP blockade with 5 µM glibenclamide impaired sensitivity pre- and post-ischemia, not eliminating the inhibitory effects of I/R. A1 adenosine receptor antagonism with 100 nM 8-cyclopentyl-1,3-dipropylxanthine worsened effects of ischemia on sensitivity. A2A adenosine receptor antagonism with 100 nM 8-(3-chlorostyryl)caffeine reduced post-ischemic flow by 50%, yet paradoxically enhanced post-ischemic contractile recovery. Conclusions: Ischemia modifies vascular control and impairs NO- versus KATP-dependent coronary dilation in an asanguinous model. Endogenous adenosine protects against vascular dysfunction via A1 receptors, and determines coronary reflow via A2A receptors. However, intrinsic A2A activation apparently worsens contractile dysfunction.

KEYWORDS Adenosine; Coronary circulation; Ischemia; KATP channel; Reperfusion; Vasoconstriction/dilation; Ventricular function


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