Skip Navigation

Cardiovascular Research 2002 55(1):104-112; doi:10.1016/S0008-6363(02)00399-1
© 2002 by European Society of Cardiology
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chatham, J. C
Right arrow Articles by Seymour, A.-M. L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chatham, J. C
Right arrow Articles by Seymour, A.-M. L
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright © 2002, European Society of Cardiology

Cardiac carbohydrate metabolism in Zucker diabetic fatty rats

John C Chathama,* and Anne-Marie L Seymourb

aCenter for NMR Research & Development, University of Alabama at Birmingham, CNIR Building, 828 Eighth Court South, Birmingham, AL 35294, USA
bDepartment of Biological Sciences, University of Hull, Cottingham Road, Hull HU6 7RX, UK

jchatham{at}uab.edu

* Corresponding author. Tel.: +1-205-934-0240; fax: +1-205-934-7367

Objective: The aim of this study was to test the hypothesis that, shortly after the development of Type-2 diabetes, alterations in cardiac carbohydrate metabolism precede the onset of abnormalities in systolic function. Methods: Hearts from 11-week-old Zucker diabetic fatty (ZDF) rats and age matched controls were perfused in the isovolumic Langendorff mode with 13C-labeled glucose, lactate and pyruvate and unlabeled fatty acids. 13C-Nuclear magnetic resonance glutamate isotopomer analysis was carried out to determine the contributions of substrates to energy production. Results: The ZDF group was hyperglycemic and the relative flux through pyruvate dehydrogenase (PDH) was significantly depressed compared to lean controls. In the lean group, lactate, pyruvate and glucose contributed 64±3, 24±3 and 11±1%, respectively, to total pyruvate oxidation. In the ZDF group, the contribution of glucose both to total pyruvate oxidation and to tissue lactate and alanine formation was significantly depressed. Cardiac function assessed by the rate-pressure product was similar in both groups. The fraction of active PDH was decreased in the ZDF group compared to controls (p<0.025). Conclusions: These results highlight significant changes in cardiac carbohydrate metabolism shortly after the development of hyperglycemia in a model of Type 2 diabetes in the absence of overt changes in systolic function.

KEYWORDS Cardiomyopathy; Contractile function; Diabetes; Energy metabolism; Glycolysis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Bonen, G. P. Holloway, N. N. Tandon, X.-X. Han, J. McFarlan, J. F. C. Glatz, and J. J. F. P. Luiken
Cardiac and skeletal muscle fatty acid transport and transporters and triacylglycerol and fatty acid oxidation in lean and Zucker diabetic fatty rats
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2009; 297(4): R1202 - R1212.
[Abstract] [Full Text] [PDF]

Home page
 DMMHome page
H. Bugger and E. D. Abel
Rodent models of diabetic cardiomyopathy
Dis. Model. Mech., September 1, 2009; 2(9-10): 454 - 466.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y.-G. Niu and R. D. Evans
Myocardial metabolism of triacylglycerol-rich lipoproteins in type 2 diabetes
J. Physiol., July 1, 2009; 587(13): 3301 - 3315.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J. J. Wright, J. Kim, J. Buchanan, S. Boudina, S. Sena, K. Bakirtzi, O. Ilkun, H. A. Theobald, R. C. Cooksey, K. V. Kandror, et al.
Mechanisms for increased myocardial fatty acid utilization following short-term high-fat feeding
Cardiovasc Res, May 1, 2009; 82(2): 351 - 360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. P. Chandler, E. E. Morgan, T. A. McElfresh, T. A. Kung, J. H. Rennison, B. D. Hoit, and M. E. Young
Heart failure progression is accelerated following myocardial infarction in type 2 diabetic rats
Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1609 - H1616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J.-Y. Deng, J.-P. Huang, L.-S. Lu, and L.-M. Hung
Impairment of cardiac insulin signaling and myocardial contractile performance in high-cholesterol/fructose-fed rats
Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H978 - H987.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Yue, T. Arai, M. Terashima, A. Y. Sheikh, F. Cao, D. Charo, G. Hoyt, R. C. Robbins, E. A. Ashley, J. Wu, et al.
Magnetic resonance imaging of progressive cardiomyopathic changes in the db/db mouse
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2106 - H2118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Belke, E. Swanson, J. Suarez, B. T. Scott, A. E. Stenbit, and W. H. Dillmann
Increased expression of SERCA in the hearts of transgenic mice results in increased oxidation of glucose
Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1755 - H1763.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. An and B. Rodrigues
Role of changes in cardiac metabolism in development of diabetic cardiomyopathy
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1489 - H1506.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. Chabowski, J. C. Chatham, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen
Fatty acid transport and FAT/CD36 are increased in red but not in white skeletal muscle of ZDF rats
Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E675 - E682.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. Boudina and E. D. Abel
Mitochondrial uncoupling: a key contributor to reduced cardiac efficiency in diabetes.
Physiology, August 1, 2006; 21: 250 - 258.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
I. G. Poornima, P. Parikh, and R. P. Shannon
Diabetic Cardiomyopathy: The Search for a Unifying Hypothesis
Circ. Res., March 17, 2006; 98(5): 596 - 605.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Buchanan, P. K. Mazumder, P. Hu, G. Chakrabarti, M. W. Roberts, U. J. Yun, R. C. Cooksey, S. E. Litwin, and E. D. Abel
Reduced Cardiac Efficiency and Altered Substrate Metabolism Precedes the Onset of Hyperglycemia and Contractile Dysfunction in Two Mouse Models of Insulin Resistance and Obesity
Endocrinology, December 1, 2005; 146(12): 5341 - 5349.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. S. Golfman, C. R. Wilson, S. Sharma, M. Burgmaier, M. E. Young, P. H. Guthrie, M. Van Arsdall, J. V. Adrogue, K. K. Brown, and H. Taegtmeyer
Activation of PPAR{gamma} enhances myocardial glucose oxidation and improves contractile function in isolated working hearts of ZDF rats
Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E328 - E336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Panagia, G. F. Gibbons, G. K. Radda, and K. Clarke
PPAR-{alpha} activation required for decreased glucose uptake and increased susceptibility to injury during ischemia
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2677 - H2683.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Wang, S. G. Lloyd, H. Zeng, A. Bonen, and J. C. Chatham
Impact of altered substrate utilization on cardiac function in isolated hearts from Zucker diabetic fatty rats
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2102 - H2110.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. D. Belke, E. A. Swanson, and W. H. Dillmann
Decreased Sarcoplasmic Reticulum Activity and Contractility in Diabetic db/db Mouse Heart
Diabetes, December 1, 2004; 53(12): 3201 - 3208.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
P. K. Mazumder, B. T. O'Neill, M. W. Roberts, J. Buchanan, U. J. Yun, R. C. Cooksey, S. Boudina, and E. D. Abel
Impaired Cardiac Efficiency and Increased Fatty Acid Oxidation in Insulin-Resistant ob/ob Mouse Hearts
Diabetes, September 1, 2004; 53(9): 2366 - 2374.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
Z. Y. Fang, J. B. Prins, and T. H. Marwick
Diabetic Cardiomyopathy: Evidence, Mechanisms, and Therapeutic Implications
Endocr. Rev., August 1, 2004; 25(4): 543 - 567.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. G. Lloyd, P. Wang, H. Zeng, and J. C. Chatham
Impact of low-flow ischemia on substrate oxidation and glycolysis in the isolated perfused rat heart
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H351 - H362.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Lajoie, A. Calderone, F. Trudeau, N. Lavoie, G. Massicotte, S. Gagnon, and L. Beliveau
Exercise training attenuated the PKB and GSK-3 dephosphorylation in the myocardium of ZDF rats
J Appl Physiol, May 1, 2004; 96(5): 1606 - 1612.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. Wang and J. C. Chatham
Onset of diabetes in Zucker diabetic fatty (ZDF) rats leads to improved recovery of function after ischemia in the isolated perfused heart
Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E725 - E736.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. N. Carley, L. M. Semeniuk, Y. Shimoni, E. Aasum, T. S. Larsen, J. P. Berger, and D. L. Severson
Treatment of type 2 diabetic db/db mice with a novel PPAR{gamma} agonist improves cardiac metabolism but not contractile function
Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E449 - E455.
[Abstract] [Full Text]

Home page
 CirculationHome page
M. Scheuermann-Freestone, P. L. Madsen, D. Manners, A. M. Blamire, R. E. Buckingham, P. Styles, G. K. Radda, S. Neubauer, and K. Clarke
Abnormal Cardiac and Skeletal Muscle Energy Metabolism in Patients With Type 2 Diabetes
Circulation, June 24, 2003; 107(24): 3040 - 3046.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Lloyd, C. Brocks, and J. C. Chatham
Differential modulation of glucose, lactate, and pyruvate oxidation by insulin and dichloroacetate in the rat heart
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H163 - H172.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
E. Aasum, A. D. Hafstad, D. L. Severson, and T. S. Larsen
Age-Dependent Changes in Metabolism, Contractile Function, and Ischemic Sensitivity in Hearts From db/db Mice
Diabetes, February 1, 2003; 52(2): 434 - 441.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.