© 2001 by European Society of Cardiology
Copyright © 2000, European Society of Cardiology
Nitric oxide synthase inhibition increases aortic stiffness measured by pulse wave velocity in rats
Department of Pharmacology, Berlex Biosciences, P.O. Box 4409, 15049 San Pablo Avenue, Richmond, CA 94804-0099, USA
* Corresponding author. Tel.: +1-510-669-4489; fax: +1-510-669-4247 jimwang{at}berlex.com
Objective: The present study was to examine whether endogenous nitric oxide (NO) plays a role in the regulation of vascular stiffness. Methods: Pulse wave velocity (PWV) was determined as the time delay between the foot of pressure waves recorded simultaneously at the aortic arch and abdominal aorta (just above the bifurcation) in anesthetized Sprague–Dawley rats. A decrease in vascular compliance results in an increase in PWV. Results: A bolus injection of a NO synthase inhibitor, L-NAME (30 mg/kg), significantly increased PWV, accompanied by an increase in blood pressure. Since changes in blood pressure are known to affect PWV, phenylephrine (PE) was administered to mimic the blood pressure changes induced by L-NAME, thus compensating for the pressure-dependent component of the PWV changes. At each given level of mean arterial pressure (MAP), PWV was significantly higher with L-NAME than with PE treatment, suggesting that acute withdrawal of endogenous NO reduces aortic compliance independent of changes in MAP. In rats chronically treated with L-NAME (0.5 g/l in drinking water) for 3 weeks, PWV was even higher than those acutely treated with L-NAME (at MAP=150 mmHg). This additional increase in vascular stiffness may be due to the remodeling of the vascular wall as a result of chronic NOS inhibition and hypertension. Conclusion: These data demonstrate that NO modulates vascular compliance independent of blood pressure changes and that an intact endogenous NO system is required to maintain normal vascular compliance.
KEYWORDS Arteries; Blood pressure; Endothelial function; Nitric oxide
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Ryoo, G. Gupta, A. Benjo, H. K. Lim, A. Camara, G. Sikka, H. K. Lim, J. Sohi, L. Santhanam, K. Soucy, et al. Endothelial Arginase II: A Novel Target for the Treatment of Atherosclerosis Circ. Res., April 25, 2008; 102(8): 923 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. F. Mitchell, C.-Y. Guo, S. Kathiresan, R. S. Vasan, M. G. Larson, J. A. Vita, M. J. Keyes, M. Vyas, C. Newton-Cheh, S. L. Musone, et al. Vascular Stiffness and Genetic Variation at the Endothelial Nitric Oxide Synthase Locus: The Framingham Heart Study Hypertension, June 1, 2007; 49(6): 1285 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cosson, M. Herisse, D. Laude, F. Thomas, P. Valensi, J.-R. Attali, M. E. Safar, and H. Dabire Aortic stiffness and pulse pressure amplification in Wistar-Kyoto and spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2506 - H2512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Soucy, S. Ryoo, A. Benjo, H. K. Lim, G. Gupta, J. S. Sohi, J. Elser, M. A. Aon, D. Nyhan, A. A. Shoukas, et al. Impaired shear stress-induced nitric oxide production through decreased NOS phosphorylation contributes to age-related vascular stiffness J Appl Physiol, December 1, 2006; 101(6): 1751 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P E Gates and D R Seals Decline in large elastic artery compliance with age: a therapeutic target for habitual exercise Br. J. Sports Med., November 1, 2006; 40(11): 897 - 899. [Full Text] [PDF]
|
|
|
|
|
|
R. M. Fitch, J. C. Rutledge, Y.-X. Wang, A. F. Powers, J.-L. Tseng, T. Clary, and G. M. Rubanyi Synergistic effect of angiotensin II and nitric oxide synthase inhibitor in increasing aortic stiffness in mice Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1190 - H1198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Naka, A. C. Tweddel, S. N. Doshi, J. Goodfellow, and A. H. Henderson Flow-mediated changes in pulse wave velocity: a new clinical measure of endothelial function Eur. Heart J., February 1, 2006; 27(3): 302 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Davies, Z. I. Whinnett, D. P. Francis, K. Willson, R. A. Foale, I. S. Malik, A. D. Hughes, K. H. Parker, and J. Mayet Use of simultaneous pressure and velocity measurements to estimate arterial wave speed at a single site in humans Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H878 - H885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. White, S. Ryoo, D. Li, H. C. Champion, J. Steppan, D. Wang, D. Nyhan, A. A. Shoukas, J. M. Hare, and D. E. Berkowitz Knockdown of Arginase I Restores NO Signaling in the Vasculature of Old Rats Hypertension, February 1, 2006; 47(2): 245 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schmitt, A. Avolio, A. Qasem, C. M. McEniery, M. Butlin, I. B. Wilkinson, and J. R. Cockcroft Basal NO Locally Modulates Human Iliac Artery Function In Vivo Hypertension, July 1, 2005; 46(1): 227 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sugawara, S. Maeda, T. Otsuki, T. Tanabe, R. Ajisaka, and M. Matsuda Effects of nitric oxide synthase inhibitor on decrease in peripheral arterial stiffness with acute low-intensity aerobic exercise Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2666 - H2669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. Wilkinson, S. S. Franklin, and J. R. Cockcroft Nitric Oxide and the Regulation of Large Artery Stiffness: From Physiology to Pharmacology Hypertension, August 1, 2004; 44(2): 112 - 116. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Iaccarino, M. Ciccarelli, D. Sorriento, E. Cipolletta, V. Cerullo, G. L. Iovino, A. Paudice, A. Elia, G. Santulli, A. Campanile, et al. AKT Participates in Endothelial Dysfunction in Hypertension Circulation, June 1, 2004; 109(21): 2587 - 2593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Eskurza, K. D. Monahan, J. A. Robinson, and D. R. Seals Ascorbic acid does not affect large elastic artery compliance or central blood pressure in young and older men Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1528 - H1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A. Massy, C. Fumeron, D. Borderie, P. Tuppin, T. Nguyen-Khoa, M.-O. Benoit, C. Jacquot, C. Buisson, T. B. Drueke, O. G. Ekindjian, et al. Increased Plasma S-Nitrosothiol Concentrations Predict Cardiovascular Outcomes among Patients with End-Stage Renal Disease: A Prospective Study J. Am. Soc. Nephrol., February 1, 2004; 15(2): 470 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Safar and P. Laurent Pulse pressure and arterial stiffness in rats: comparison with humans Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1363 - H1369. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lerman and J. Herrmann Endothelial function under pressure J. Am. Coll. Cardiol., May 21, 2003; 41(10): 1759 - 1760. [Full Text] [PDF]
|
|
|
|
 |
|
 B. N Van Vliet, L. L Chafe, and J.-P. Montani Characteristics of 24 h Telemetered Blood Pressure in eNOS-Knockout and C57Bl/6J Control Mice J. Physiol., May 15, 2003; 549(1): 313 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Mitchell, J. L. Izzo Jr, Y. Lacourciere, J.-P. Ouellet, J. Neutel, C. Qian, L. J. Kerwin, A. J. Block, and M. A. Pfeffer Omapatrilat Reduces Pulse Pressure and Proximal Aortic Stiffness in Patients With Systolic Hypertension: Results of the Conduit Hemodynamics of Omapatrilat International Research Study Circulation, June 25, 2002; 105(25): 2955 - 2961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Cabrera Fischer, R. L. Armentano, F. M. Pessana, S. Graf, L. Romero, A. I. Christen, A. Simon, and J. Levenson Endothelium-dependent arterial wall tone elasticity modulated by blood viscosity Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H389 - H394. [Abstract] [Full Text] [PDF]
|
|