© 2001 by European Society of Cardiology
Copyright © 2000, European Society of Cardiology
Postischemic myocardial recovery and oxidative stress status of vitamin C deficient rat hearts
aLaboratoire de Physiopathologie et Pharmacologie Cardio-vasculaires Expérimentales, Facultés de Médecine et Pharmacie, 7 Boulevard Jeanne d’Arc, 21000 Dijon, France
bLaboratoire de Physiologie, Facultés de Médecine and Pharmacie, Dijon, France
* Corresponding author. Tel.: +33-380-393-292; fax: +33-380-393-293 catherine.vergely{at}u-bourgogne.fr
Objective: To investigate the role of vitamin C tissue content as a protective agent during myocardial ischemia–reperfusion injury, we have evaluated the postischemic functional recovery and free radical release of osteogenic disorder Shionogi (ODS) inherently scorbutic rat hearts and compared them to healthy Wistar rat hearts. Methods: Isolated perfused hearts of ODS or Wistar rats underwent 30 min of a global total normothermic ischemia followed by 30 min of reperfusion. The lipid-soluble spin trap 
-phenyl N-tert-butylnitrone (3 mM) was perfused upstream of the coronary bed. Functional parameters were recorded and samples of coronary effluents were analysed using electron spin resonance spectroscopy to characterise and quantify the amount of radical species released. Results: From the onset of reperfusion, a large and long-lasting release of alkyl/alkoxyl radicals was detected, with a peak value of 29.0±3.2 nM obtained after 13 min, which was associated with a persistent contractile dysfunction. However, ODS rat hearts showed a higher myocardial recovery with lower left ventricular end diastolic pressure (44.34±1.74 vs. 55.03±1.57 mmHg for Wistar), higher recovery of rate pressure product (12.3±1.4 vs. 1.9±1.7x103 mmHgbeats/min for Wistar) and shorter duration of contractile abnormalities during reperfusion (3.7±1.0 vs. 20.8±5.3 min for Wistar). Moreover, free radical release was identical in ODS rat hearts as compared to control Wistar rats. Ascorbic acid tissue content was significantly altered in ODS rats (31.9±3.3 vs. 591.0±54.9 mmol/g of tissue for Wistar) but superoxide dismutases, glutathion peroxidases and inducible heat shock protein 70 genes were up-regulated. Conclusions: This study shows that ascorbic-acid-deficient ODS rat hearts are more resistant to an ischemic insult than control Wistar rats, probably through the development of alternative protective defences, like the induction of heat shock proteins. These paradoxical results raise the question of the relative importance of each endogenous antioxidant in the cardiac resistance to ischemia–reperfusion injury.
KEYWORDS Free radicals; Gene expression; Ischemia; NMR; Reperfusion; Ventricular function
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Vergely, F. Goirand, A. Ecarnot-Laubriet, C. Renard, D. Moreau, J.-C. Guilland, M. Dumas, and L. Rochette Vitamin C Deficiency Exerts Paradoxical Cardiovascular Effects in Osteogenic Disorder Shionogi (ODS) Rats J. Nutr., April 1, 2004; 134(4): 729 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vergely, C. Perrin-Sarrado, G. Clermont, and L. Rochette Postischemic Recovery and Oxidative Stress Are Independent of Nitric-Oxide Synthases Modulation in Isolated Rat Heart J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 149 - 157. [Abstract] [Full Text] [PDF]
|
|