© 2001 by European Society of Cardiology
Copyright © 2000, European Society of Cardiology
Reduction in density of transverse tubules and L-type Ca2+ channels in canine tachycardia-induced heart failure
aDepartment of Medicine, University of Wisconsin, Madison, Wisconsin, WI 53792, USA
bDepartment of Physiology, University of Wisconsin, Madison, Wisconsin, WI 53792, USA
cDepartment of Surgery, University of Wisconsin, Madison, Wisconsin, WI 53792, USA
* Corresponding author. Tel.: +1-608-263-4856; fax: +1-608-263-0405 tjk{at}medicine.wisc.edu
Objective: Persistent supraventricular tachycardia leads to the development of a dilated cardiomyopathy with impairment of excitation–contraction (EC) coupling. Since the initial trigger for EC coupling in ventricular muscle is the influx of Ca2+ through L-type Ca2+ channels (ICa) in the transverse tubules (T-tubules), we determined if the density of the T-tubule system and L-type Ca2+ channels change in canine tachycardia pacing-induced cardiomyopathy. Methods: Confocal imaging of isolated ventricular myocytes stained with the membrane dye Di-8-ANEPPS was used to image the T-tubule system, and standard whole-cell patch clamp techniques were used to measure ICa and intramembrane charge movement. Results: A complex staining pattern of interconnected tubules including prominent transverse components spaced every 
1.6 µm was present in control ventricular myocytes, but failing cells demonstrated a far less regular T-tubule system with a relative loss of T-tubules. In confocal optical slices, the average % of the total cell area staining for T-tubules decreased from 11.5±0.4 in control to 8.7±0.4% in failing cells (P<0.001). Whole-cell patch clamp studies revealed that ICa density was unchanged. Since whole-cell ICa is due to both the number of channels as well as the functional properties of those channels, we measured intramembrane charge movement as an assay for changes in channel number. The saturating amount of charge that moves due to gating of L-type Ca2+ channels, Qon,max, was decreased from 6.5±0.6 in control to 2.8±0.3 fC/pF in failing myocytes (P<0.001). Conclusions: Cellular remodeling in heart failure results in decreased density of T-tubules and L-type Ca2+ channels, which contribute to abnormal EC coupling.
KEYWORDS Ca-channel; e–c coupling; Heart failure; Myocytes; Remodeling
1 These authors contributed equally to this work.
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